Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2) antibody

Antigen: Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2)

Clonality: Monoclonal (TX31)

Host: Rat

Application: Flow Cytometry (FACS)

Catalog no.: ABIN130699

Additional Information

Alternative name: CD112/Nectin-2/PRR2

Immunogen: This antibody was purified from hybridoma (clone TX31) supernatant using protein G agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Wister rat lymphocyte immunized with a human recombinant CD112.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: TX31

Description: CD112, also known as Poliovirus Receptor Related 2 (PRR2), is a transmembrane glycoprotein that functions as the receptor for alphaherpes virus and pseudorabies virus entry into the cell. CD112 has been shown to be identical to Nectin-2, an adhesion protein localized to adherens junctions. CD112 is widely expressed in neuronal, endothelial, and in particular, epithelial cells, where it is found at the intercellular junctions of adjacent cells. Additionally CD112 is detected on hematopoietic progenitors expressing CD34, where it plays a role in the regulation of hematopoietic/endothelial cell interactions. At least two isoforms of CD112 mRNA have been identified.

Specificity: This antibody reacts with human CD112 on Flow cytometry.

Application Details

Application Notes: Western blotting: Not tested. Immunoprecipitation: Not tested. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow Cytometry: 5-10 µg / mL (final concentration)

Concentration: 1mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives. 172-3 CD226 (TX25) ProtocolS:Flow cytometric analysis for floating cells Protocol 1 We usually use Fisher tubes or equivalents as reaction tubes for all steps d

Research Area: CD Antigens

Restrictions: For Research Use only

Images

Flow cytometric analysis of CD112 expression on KG -1 cells. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of ABIN130699 to the cells.

Publications

Publications: Bottino, Castriconi, Pende et al.: "Identification of PVR (CD155) and Nectin-2 (CD112) as cell surface ligands for the human DNAM-1 (CD226) activating molecule." in: The Journal of experimental medicine, Vol. 198, Issue 4, pp. 557-67, 2003 (PubMed).