Histone H1 antibody

Antigen: Histone H1

Synonyms: H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4

Clonality: Monoclonal (C14093)

Host: Mouse

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN130741

Additional Information

Immunogen: This antibody was purified from hybridoma (clone C14093) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: C14093

Description: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. Histone H1, also known as linker histone, is bound to linker DNA region between nucleosomes, contributing to chromatin foldings in the nucleus. It is known that histone H1 is also post-translationally modified, such as phosphorylation or methylation and relation of these modifications with biological meanings are under extensive investigations. Histone H1 is essential for higher order structure of mitotic chromosomes that are condensed structure of chromatin during mitosis.

Specificity: This antibody reacts with Histone H1 (32kDa) on Western blotting. .

Application Details

Application Notes: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 10 µg / 200 µL of cell extract from 5x10 6 cells. Immunocytochemistry: 1 µg / mL

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.

Research Area: DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of Histone H1 expression in HeLa cells (1), HL60 cells (2), A431 cells (3), WR19L cells (4) and PC12 cells (5) using ABIN130741.

Immunoprecipitation of Histone H1 from HeLa cells with mouse IgG2b (1) or ABIN130741 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN130741.

Publications

Publications: Vettese-Dadey, Grant, Hebbes et al.: "Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro." in: The EMBO journal, Vol. 15, Issue 10, pp. 2508-18, 1996 (PubMed).

Uchiyama, Kobayashi, Takata et al.: "Proteome analysis of human metaphase chromosomes." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 16994-7004, 2005 (PubMed).

Maresca, Freedman, Heald: "Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts." in: The Journal of cell biology, Vol. 169, Issue 6, pp. 859-69, 2005 (PubMed).

Higashi, Miyakawa, Uchiyama et al.: "Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse." in: Journal of biotechnology, Vol. 120, Issue 3, pp. 262-72, 2005 (PubMed).