Histone H2B antibody

Antigen: Histone H2B

Synonyms: DmelCG17949, CG17949, H2B, FLJ35099, FLJ56780, FLJ56787, MGC131639, HISTONE 2B, HISTONE H2B, HTB9, HTB2, MRN17.11, MRN17_11

Clonality: Monoclonal (C14264)

Host: Mouse

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN130743

Additional Information

Immunogen: This antibody was purified from hybridoma (clone C14264) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: C14264

Description: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-translational modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H2B was suppressed to greater extent than that of the others, with histone H3 being relatively least affected. The analysis of acetylation status of each of the four lysine sites at the N-terminal tail of histone H2B revealed decreases consistent with those observed in the total acetylation patterns, with the K12 and K20 residues being markedly more affected than K5 and K15 residues.

Specificity: This antibody reacts with Histone H2B (14kDa) on Western blotting. .

Application Details

Application Notes: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 200 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: 1 µg / mL. Flow Cytometry: Not tested.

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.

Research Area: DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of Histone H2B expression in Jurkat cells (1), HeLa cells (2), Raji cells (3), HL60 cells (4), A431 cells (5), L5178Y cells (6), WR19L cells (7), NIH/3T3 cells (8), CHO cells (9), PC12 cells (10) and Rat1 cells (11) using ABIN130743.

Immunoprecipitation of Histone H2B from HeLa cells with mouse IgG2b (1) or ABIN130743 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN130743.

Immunocytochemical detection of Histone H2B on 4% PFA fixed HeLa cells with ABIN130743.

Publications

Publications: Vettese-Dadey, Grant, Hebbes et al.: "Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro." in: The EMBO journal, Vol. 15, Issue 10, pp. 2508-18, 1996 (PubMed).

Uchiyama, Kobayashi, Takata et al.: "Proteome analysis of human metaphase chromosomes." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 16994-7004, 2005 (PubMed).

Higashi, Miyakawa, Uchiyama et al.: "Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse." in: Journal of biotechnology, Vol. 120, Issue 3, pp. 262-72, 2005 (PubMed).