Histone H4 (HIST2H4A) antibody

Antigen: Histone H4 (HIST2H4A)

Synonyms: H4, H4/n, H4F2, H4FN, FO108, HIST2H4, H4/c, H4FC, HIST4H4, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, H4/p, HIST1H4F, MGC24116, DmelCG31611, CG31611, Hist4h4, Hist1h4a, Hist1h4b, Hist1h4d, Hist1h4f, Hist1h4h, Hist1h4i, Hist1h4j, Hist1h4k, Hist1h4m, Hist2h4a, Hist1h4c, B130044J01Rik, F8N16.2, F8N16_2, HIS4, HISTONE H4, F16L2.140, F18L15.40, F5K20.30, MTH12.15, MMN10.3, F24B9.25, F24B9.8, h4, gb:am422106

Clonality: Monoclonal (C14691)

Host: Mouse

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN130744

Additional Information

Alternative name: Histone H4

Immunogen: This antibody was purified from hybridoma (clone C14691) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: C14691

Description: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes multiple different post-transcriptional modifications including acetylation, phosphorylation, ubiquitination, methylation, and ADP-ribosylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. Acetylation of histone H4 plays a primary role in the structural changes that mediate enhanced binding of transcription factors to their recognition sites within nucleosomes. It has been suggested that acetylated Histone H4 also is involved in heightened activation of the transcription of male X chromosomes, and that Histone H4 stimulates glucose transport activity in rat skeletal muscle.

Specificity: This antibody reacts with Histone H4 (11kDa) on Western blotting. .

Synonyms: H4, H4/n, H4F2, H4FN, FO108, HIST2H4, H4/c, H4FC, HIST4H4, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4A, HIST2H4B, H4/p, HIST1H4F, MGC24116, DmelCG31611, CG31611, Hist4h4, Hist1h4a, Hist1h4b, Hist1h4d, Hist1h4f, Hist1h4h, Hist1h4i, Hist1h4j, Hist1h4k, Hist1h4m, Hist2h4a, Hist1h4c, B130044J01Rik, F8N16.2, F8N16_2, HIS4, HISTONE H4, F16L2.140, F18L15.40, F5K20.30, MTH12.15, MMN10.3, F24B9.25, F24B9.8, h4, gb:am422106

Application Details

Application Notes: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 5 µg / 200 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: 5 µg / mL. Flow Cytometry: Not tested.

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.

Research Area: DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of Histone H4 expression in HeLa cells (1), Raji cells (2), HL-60 cells (3), A431 cells (4), L5178Y cells (5), WR19L cells (6), NIH/3T3 cells (7), CHO cells (8), PC12 cells (9) and Rat1 cells (10) using ABIN130744.

Immunoprecipitation of Histone H4 from HeLa cells with Mouse IgG2b (1) or ABIN130744 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDSPAGE and immunoblotted with ABIN130744.

Immunocytochemical detection of Histone H4 on 4% PFA fixed HeLa cells with ABIN130744.

Publications

Publications: Vettese-Dadey, Grant, Hebbes et al.: "Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro." in: The EMBO journal, Vol. 15, Issue 10, pp. 2508-18, 1996 (PubMed).

Uchiyama, Kobayashi, Takata et al.: "Proteome analysis of human metaphase chromosomes." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 16994-7004, 2005 (PubMed).

Higashi, Miyakawa, Uchiyama et al.: "Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse." in: Journal of biotechnology, Vol. 120, Issue 3, pp. 262-72, 2005 (PubMed).