Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRNPA2B1) antibody

Antigen: Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRNPA2B1)

Synonyms: RNPA2, HNRPA2, HNRPB1, SNRPB1, HNRNPA2, HNRNPB1, FLJ22720, HNRPA2B1, DKFZp779B0244, hnrpa2b1, MGC53135, HNRNPA2B1, rnpa2, hnrpa2, hnrpb1, snrpb1, hnrnpa2, hnrnpb1, MGC147698

Clonality: Monoclonal (C20308)

Host: Mouse

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN130746

Additional Information

Alternative name: HnRNP-A2/B1

Immunogen: This antibody was purified from hybridoma (clone C20308) ascites using ImmunoPure IgM Purification Kit (PIERCE: Product no. 44897). This hybridoma was established by fusion of mouse myeloma cell Sp2/0-Ag14 with Balb/c mouse splenocyte immunized with the protein fraction extracted from the mitotic chromosome.

Isotype: IgM, IgG  (Matching secondary antibodies)

Clone: C20308

Description: The heterogeneous nuclear ribonucleoprotein-A2/B1 (hnRNP-A2/B1) was recently shown to be up-regulated in the early stage of lung cancer. This protein plays an important role in biogenesis and transport of mRNA. Up-regulation of hnRNP usually precedes morphological differentiation and is considered a good biomarker in the early stages of cancer development. HnRNP-A2/B1 proteins were localized primarily in the nucleus, excluding the nucleolus, although the expression of A2 protein was weaker than that of B1 protein. hnRNP- A2/B1 proteins were expressed in nonmalignant alveolar cells as well as cancer cells, although the expression in alveolar cells was weaker than that of cancer cells.

Specificity: This antibody reacts with hnRNP-A2/B1 (40kDa) on Western blotting. .

Application Details

Application Notes: Western blotting: 10 µg / mL for chemiluminescence detection system. Immunoprecipitation: Not tested. Immunohistochemistry: Not tested. Immunocytochemistry: 10 µg / mL. Flow Cytometry: Not tested.

Concentration: 1 mg/mL

Buffer: 100 µg IgM in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives.

Research Area: DNA/RNA, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of hnRNPA2/ B1 expression in Jurkat cells (1), Raji cells (2), HeLa cells (3), MCF-7 cells (4) and NIH/3T3 cells (5) using ABIN130746.

Immunocytochemical detection of hnRNP-A2/B1 on acetone fixed HEp-? cells with ABIN130746.

Publications

Publications: Vettese-Dadey, Grant, Hebbes et al.: "Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro." in: The EMBO journal, Vol. 15, Issue 10, pp. 2508-18, 1996 (PubMed).

Uchiyama, Kobayashi, Takata et al.: "Proteome analysis of human metaphase chromosomes." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 16994-7004, 2005 (PubMed).

Maresca, Freedman, Heald: "Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts." in: The Journal of cell biology, Vol. 169, Issue 6, pp. 859-69, 2005 (PubMed).

Higashi, Miyakawa, Uchiyama et al.: "Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse." in: Journal of biotechnology, Vol. 120, Issue 3, pp. 262-72, 2005 (PubMed).