ADAM Metallopeptidase Domain 10 (ADAM10) antibody

Antigen: ADAM Metallopeptidase Domain 10 (ADAM10)

Synonyms: kuz, AD10, MADM, CD156c, HsT18717, kuzbanian, 1700031C13Rik, ADAM10, adam10, wu:fc03d12, ADAM-10, DKFZp459A157, LOC100219653, Adam10

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN130854

Additional Information

Alternative name: ADAM10

Isotype: IgG, Ig Fraction  (Matching secondary antibodies)

Description: Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for A Disintegrin And Metalloprotease), was recently identified to cleave TNF-a, Notch, and its ligand Delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.

Specificity: The antibody detects 85 kDa precursor form and 60 kDa processed mature form of ADAM10.

Application Details

Application Notes: The antibody can be used for Western blotting (1 µg/ml). However, the optimal conditions should be determined individually.

Buffer: 100 µg (0.5 mg/ml) affinity purified rabbit anti-ADAM10 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Research Area: Proteases, Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Pan, Rubin: "Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis." in: Cell, Vol. 90, Issue 2, pp. 271-80, 1997 (PubMed).

Rosendahl, Ko, Long et al.: "Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases." in: The Journal of biological chemistry, Vol. 272, Issue 39, pp. 24588-93, 1997 (PubMed).