RasGAP antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN130897 |
| Quantity |
100 µg |
| Price |
Product not available in this region.
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| Shipping to |
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Isotype
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IgG, Ig Fraction (Matching secondary antibodies)
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Description
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The mammalian proto-oncogene Ras encodes 21 kDa proteins (p21Ras) which exists in either a physiologically quiescent GDP-binding state or a GTP binding signal-emitting state. Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold. The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity.
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Specificity
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The antibody recognizes ~120 kDa Ras GAP of human, mouse, and rat origins. Reactivity to other species has not been tested.
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Application Notes
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The antibody can be used for Western blotting (1-4 µg/ml), immunoprecipitation (20 µg/ml) and immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually.
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Buffer
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Supplied as 100 µg (0.2 mg/ml) rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.
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Restrictions
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For Research Use only
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Alternatives for antigen "RasGAP", type "Antibodies"
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Hosts
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Rabbit (16)
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Reactivities
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Human (16), Mouse (Murine) (16), Rat (Rattus) (16)
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Applications
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Flow Cytometry (FACS) (13), Immunofluorescence (IF) (13), Western Blotting (WB) (4), ELISA (3), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoelectron Microscopy (EM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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