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Jun antibody

Antigen

Jun
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN131235
Quantity 100 µg
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives Secondary Antibodies back to top

Isotype IgG, Ig Fraction  (Matching secondary antibodies)
Description Extracellular stimuli such as growth factors and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation at Ser63/73 renders c-Jun non-responsive to mitogenic and stress induced signaling pathways. JNK (c-jun N-terminal Kinase) binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. The activity of JNK is stimulated by the same signals that activate c-Jun.
Specificity Recognizes c-Jun in samples of human, mouse, and rat origin. The antibody only recognizes c-Jun, and will not detect the endogenous levels of Jun B or Jun D.

Application Details
Publications Alternatives Secondary Antibodies back to top

Application Notes The antibody can be used for Western blot analysis (1 µg/ml) and immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually.
Buffer Supplied as 100 µg (0.5 mg/ml) affinity purified rabbit anti-c-Jun polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
Restrictions For Research Use only

Publications
Application Details Alternatives Secondary Antibodies back to top

Publications Binetruy, Smeal, Karin: "Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain." in: Nature, Vol. 351, Issue 6322, pp. 122-7, 1991 (PubMed).

Derijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).