GM-CSF antibody
| Antigen |
|
| Synonyms |
GMCSF, GM-CSF |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
Western Blotting (WB) (68), Enzyme Immunoassay (EIA) (54), Flow Cytometry (FACS) (32), ELISA (31), Immunofluorescence (IF) (30), Functional Studies (Func) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (Detection) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Neutralization (Neut) (4), Radioimmunoassay (RIA) (4), Blocking Antibody (Inhibition) (3), ELISPOT (3), Immunoelectron Microscopy (EM) (2)
|
| Catalog no. |
ABIN131314 |
| Quantity |
100 µg (Variants) |
| Price |
Product not available in this region.
|
| Shipping to |
|
|
Immunogen
|
Immunogen used was recombinant human GM-CSF expressed in E. coli.
|
|
Isotype
|
IgG, Ig Fraction (Matching secondary antibodies)
|
|
Description
|
Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. Human GM-CSF is a 14.0 kDa protein containing 123 amino acid residues.
|
|
Application Notes
|
The antibody can be used for Western blot analysis (0.5-1 µg/ml), ELISA (2-3 µg/ml), and neutralization assays (1-5 µg/ml). However, the optimal conditions should be determined individually.
|
|
Purification
|
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human GM-CSF. The antibody was purified by affinity chromatography employing immobilized human GM-CSF matrix.
|
|
Buffer
|
Supplied as 100 µg (0.5 mg/ml) affinity purified rabbit anti-human GM-CSF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "GM-CSF", type "Antibodies"
|
Hosts
|
Rabbit (59), Mouse (40), Rat (26), Sheep (1)
|
|
Reactivities
|
Human (71), Mouse (Murine) (38), Rat (Rattus) (19), Cow (Bovine) (18), Chicken (15), Dog (Canine) (15), Pig (Porcine) (15), Sheep (Ovine) (2), Macaque (1), Monkey (1)
|
|
Applications
|
Western Blotting (WB) (68), Enzyme Immunoassay (EIA) (54), Flow Cytometry (FACS) (32), ELISA (31), Immunofluorescence (IF) (30), Functional Studies (Func) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (Detection) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Neutralization (Neut) (4), Radioimmunoassay (RIA) (4), Blocking Antibody (Inhibition) (3), ELISPOT (3), Immunoelectron Microscopy (EM) (2)
|
|
Conjugates
|
Biotin (14), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
|
Alternatives 
