Kaiso antibody

Antigen: Kaiso

Clonality: Monoclonal (11D7)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN131594

Additional Information

Immunogen: This antibody was purified from hybridoma (clone 11D7) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell SP2/0 with A/J mouse splenocyte immunized with the 6xHis tagged Kaiso antigen containing a.a.1 through a.a.499.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 11D7

Description: Kaiso is a member of the POZ-zinc finger family of transcription factors implicated in development and cancer. It acts as a DNA methylation-dependent transcriptional repressor. Kaiso is a dual specificity DNA-binding protein that recognizes the specific consensus sequence CTGCNA as well as methyl-CpG dinucleotides. Two copies of the Kaiso-binding site are present in the matrilysin promoter, implicating matrilysin as a candidate target gene for Kaiso. Kaiso also associates with the adhesion molecule p120 ctn and may be a downstream effector of cadherin and/or p120 ctn signaling. The DNA-binding ability of Kaiso with either recognition site was inhibited by p120 ctn . Thus, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing.

Specificity: This antibody reacts with human, mouse and rat Kaiso.

Application Details

Application Notes: Western blotting: 5 µg/mL. Immunoprecipitation: 2 µg / 200-300 µL of cell extract Immunohistochemistry: Not recommended.

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Restrictions: For Research Use only

Images

Immunoprecipitation of Kaiso from Hela cells (A) or A431 cells (B) with normal mouse IgG (1) or ABIN131594 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131594.

Western blot analysis of Kaiso expression in Hela cells (A-1), A431 cells (A-2), C2C12 cells (B-1), NIH/3T3 cells (B-2) and Rat-1 cells (B-3) using ABIN131594.

Publications

Publications: Daniel, Reynolds: "The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor." in: Molecular and cellular biology, Vol. 19, Issue 5, pp. 3614-23, 1999 (PubMed).

Daniel, Ireton, Reynolds: "Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding protein." in: Hybridoma, Vol. 20, Issue 3, pp. 159-66, 2001 (PubMed).