Catenin delta 1 (p120 Protein) (CTNND1) antibody

Antigen: Catenin delta 1 (p120 Protein) (CTNND1)

Clonality: Monoclonal (6H11)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Catalog no.: ABIN131596

Additional Information

Alternative name: p120ctn

Immunogen: This antibody was purified from hybridoma (clone 6H11) supernatant using protein-A Sepharose. This hybridoma was established by fusion of mouse myeloma cell Ag8.653 with A/J mouse splenocyte immunized with the purified 6xHis-fusion to N-terminal end of p120(amino acids 3-349 of p120 ctn type 1A) protein.

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6H11

Description: P120 ctn (formerly p120 cas or pp120) is a cytoplasmic protein that is a member of the catenin family. Originally identified as a Src-tyrosine kinase substrate, the cadherin-binding p120 ctn is associated with cell-cell adhesion via catenin/cadherin complexes in adherence junctions. p120 ctn affects cell motility via modulation of activity of Rho-family GTPases, including RhoA, Rac and Cdc42, suggesting a mechanism for regulating the balance between adhesive and motile cellular phenotypes. Due to alternative splicing and multiple translation initiation codons, several p120 ctn isoforms can be expressed from a single gene. All p120 ctn isoforms share a central Armadillo repeat domain but have divergent N- and C-termini.

Specificity: This antibody reacts with human, mouse and rat p120 ctn N-terminal epitope.

Application Details

Application Notes: Western blotting: 1 µg/mL. Immunoprecipitation: 2 µg of /200-300µL of cell extract Immunohistochemistry: Not recommended.

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Research Area: Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of p120ctn expression in A431 cells (A), Rat-1 cells (B-1) and NIH/3T3 cells (B-2) using ABIN131596.

Immunoprecipitation of p120ctn from A431 cells with normal mouse IgG (1) or ABIN131596 (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with ABIN131596.

Publications

Publications: Wu, Mariner, Thoreson et al.: "Production and characterization of monoclonal antibodies to the catenin p120ctn." in: Hybridoma, Vol. 17, Issue 2, pp. 175-83, 1998 (PubMed).

Daniel, Reynolds: "The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor." in: Molecular and cellular biology, Vol. 19, Issue 5, pp. 3614-23, 1999 (PubMed).