CFTR antibody

Antigen: CFTR

Clonality: Monoclonal (GA1)

Host: Mouse

Application: Western Blotting (WB)

Catalog no.: ABIN131704

Additional Information

Immunogen: This antibody was purified from hybridoma (clone GA1) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Ag8-653 with Balb/c mouse lymphocyte immunized with human recombinant CFTR C-terminal fragment (1387a.a.-1480a.a.).

Isotype: IgG1  (Matching secondary antibodies)

Clone: GA1

Description: Cystic Fibrosis (CF) is a common lethal genetic disease characterized by chronic bronchopulmonary disease, elevated sweat electrolytes and insufficient pancreatic function. CF is caused by mutations of the CFTR gene, which encodes the cystic fibrosis transmembrane conductance regulator (CFTR). The CFTR protein is a cAMP-activated chloride channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 which results in abnormal chloride transport. CFTR is structurally similar to multi-drug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Specificity: This antibody reacts with CFTR (200kDa) by Western blotting.

Application Details

Application Notes: Western blotting: 10 µg / mL for chemiluminescence detection system. Immunoprecipitation: Not recommended.

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL volume of PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

Restrictions: For Research Use only

Images

Western blot analysis of CFTR expression in ZR75- 1 cells (1) and MCF7 cells (2) using ABIN131704 .

Publications

Publications: Cormet-Boyaka, Di, Chang et al.: "CFTR chloride channels are regulated by a SNAP-23/syntaxin 1A complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 19, pp. 12477-82, 2002 (PubMed).

Naren, Cobb, Li et al.: "A macromolecular complex of beta 2 adrenergic receptor, CFTR, and ezrin/radixin/moesin-binding phosphoprotein 50 is regulated by PKA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 342-6, 2003 (PubMed).

Li, Roy, Dandridge et al.: "Molecular assembly of cystic fibrosis transmembrane conductance regulator in plasma membrane." in: The Journal of biological chemistry, Vol. 279, Issue 23, pp. 24673-84, 2004 (PubMed).