Cohesin (Scc1/Rad21) antibody

Overview


	Antigen:	Cohesin (Scc1/Rad21)
	Antibody Type:	Monoclonal
	Application:	Western Blotting (WB), Immunoprecipitation (IP)
	Host:	Mouse
	Quantity:	100 µg (1 mg/mL)
	Price:	241,00 € (Plus shipping costs , €10.00 dry ice fee and VAT)
	Order number:	ABIN131782
	Availability:	7 to 10 days until shipment

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Additional Information: Cohesin (Scc1/Rad21) antibody

Antigen	Cohesin (Scc1/Rad21)
Immunogen	This antibody was purified from hybridoma (clone CM110-2C10) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with human recombinant Cohesin C-terminal fragment (544 a.a. – 631 a.a.)
Antibody Type	Monoclonal
Isotype	IgG1 »Matching secondary antibodies
Description	The cohesin complex is required for tethering sister chromatids after DNA replication to make certain that genomic information is replicated accurately. Cohesin consists of four proteins: Smc1, Smc3, Scc1/Mcd1, which is called kleisin and is the target of the protease separase, and Scc3/Irr1. The cohesin complex apparently forms a large ring within which sister chromatids are trapped. As the cellular division process continues, separase destroys the cohesin complex by means of cleaving its Scc1 subunit, allowing the chromatids to separate and divide with the cell. The cohesin complex may also play a role in spindle pole assembly during mitosis and prevents splitting of chromosomes once bipolar attachments are made.
Clone	CM110-2C10
Host	Mouse
Specificity	This antibody reacts with Cohesin (120kDa) on Western blotting.

Application Details: Cohesin (Scc1/Rad21) antibody

Application	Western Blotting (WB), Immunoprecipitation (IP)
Application Notes	Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 100 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow cytometry: Not tested.
Concentration	1 mg/mL
Buffer	100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives. 0090-3 Anti-Securin/Pds-1 (DCS-280)
Restrictions	For Research Use Only

Publications: Cohesin (Scc1/Rad21) antibody

Publications

Hoque, Ishikawa:
"Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres."
In: The Journal of biological chemistry , Vol. 276, Issue 7, pp. 5059-67, 2001/01 (PubMed)

Images: Cohesin (Scc1/Rad21) antibody

Western blot analysis of Cohesin expression in Jurkat cells (1), Raji cells (2), HeLa cells (3), NIH/3T3 cells (4) and PC12 cells (5) using ABIN131782.

Image for Cohesin (Scc1/Rad21) antibody (1)

External Information: Cohesin (Scc1/Rad21) antibody

Google Scholar	Find more references for clone CM110-2C10 on Google Scholar™
EMBL Harvester	Search human, mouse or rat genome for antigen Cohesin (Scc1/Rad21) (Bioinformatic Harvester (EMBL))