Cohesin (Scc1/Rad21) antibody

Antigen: Cohesin

Synonyms: CG3423, DmelCG3423, DmSA, DSA, DSA1, ESTS:92H2T, Scc3, SCC3

Epitope: Scc1/Rad21

Clonality: Monoclonal (CM110-2C10)

Host: Mouse

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN131782

Additional Information

Alternative name: Cohesin (Scc1/Rad21)

Immunogen: This antibody was purified from hybridoma (clone CM110-2C10) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell Sp2/0 with Balb/c mouse splenocyte immunized with human recombinant Cohesin C-terminal fragment (544 a.a. – 631 a.a.)

Isotype: IgG1  (Matching secondary antibodies)

Clone: CM110-2C10

Description: The cohesin complex is required for tethering sister chromatids after DNA replication to make certain that genomic information is replicated accurately. Cohesin consists of four proteins: Smc1, Smc3, Scc1/Mcd1, which is called kleisin and is the target of the protease separase, and Scc3/Irr1. The cohesin complex apparently forms a large ring within which sister chromatids are trapped. As the cellular division process continues, separase destroys the cohesin complex by means of cleaving its Scc1 subunit, allowing the chromatids to separate and divide with the cell. The cohesin complex may also play a role in spindle pole assembly during mitosis and prevents splitting of chromosomes once bipolar attachments are made.

Specificity: This antibody reacts with Cohesin (120kDa) on Western blotting.

Application Details

Application Notes: Western blotting: 1 µg / mL for chemiluminescence detection system. Immunoprecipitation: 2 µg / 100 µL of cell extract from 5x10 6 cells. Immunohistochemistry: Not tested. Immunocytochemistry: Not tested. Flow cytometry: Not tested.

Concentration: 1 mg/mL

Buffer: 100 µg IgG in 100 µL P BS containing 50% glycerol, pH 7.2. Contains no preservatives. 0090-3 Anti-Securin/Pds-1 (DCS-280)

Restrictions: For Research Use only

Images

Western blot analysis of Cohesin expression in Jurkat cells (1), Raji cells (2), HeLa cells (3), NIH/3T3 cells (4) and PC12 cells (5) using ABIN131782.

Publications

Publications: Hoque, Ishikawa: "Human chromatid cohesin component hRad21 is phosphorylated in M phase and associated with metaphase centromeres." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 5059-67, 2001 (PubMed).