Riboflavin Kinase Protein (RFK) (AA 1-162) (GST tag)
(1 reference)-
- Target See all Riboflavin Kinase (RFK) Proteins
- Riboflavin Kinase (RFK)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-162
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Riboflavin Kinase protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- RFK (Human) Recombinant Protein (P01)
- Sequence
- MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMN GH
- Characteristics
- Human RFK full-length ORF ( AAH07069, 1 a.a. - 162 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Riboflavin Kinase (RFK) (AA 53-162) protein (GST tag)
RFK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Riboflavin Kinase (RFK) (AA 1-162) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Riboflavin Kinase (RFK) protein (His tag)RFK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by reducing SDS-PAGE.
Riboflavin Kinase (RFK) (AA 1-155) protein (Strep Tag)Crystallography grade RFK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Riboflavin Kinase (RFK) (AA 1-155) protein (Strep Tag)Crystallography grade RFK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Riboflavin Kinase (RFK) protein (Myc-DYKDDDDK Tag)RFK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "A bifunctional molecule as an artificial flavin mononucleotide cyclase and a chemosensor for selective fluorescent detection of flavins." in: Journal of the American Chemical Society, Vol. 131, Issue 29, pp. 10107-12, (2009) (PubMed).
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: "A bifunctional molecule as an artificial flavin mononucleotide cyclase and a chemosensor for selective fluorescent detection of flavins." in: Journal of the American Chemical Society, Vol. 131, Issue 29, pp. 10107-12, (2009) (PubMed).
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- Target
- Riboflavin Kinase (RFK)
- Alternative Name
- RFK (RFK Products)
- Synonyms
- RFK Protein, 0610038L10Rik Protein, AF031381 Protein, KOI-4 Protein, RIFK Protein, riboflavin kinase Protein, FMN adenylyltransferase Protein, Riboflavin kinase Protein, RFK Protein, ribF Protein, CNC05850 Protein, Nitsa_1631 Protein, Isop_1070 Protein, Rfk Protein
- Background
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Full Gene Name: riboflavin kinase
Synonyms: FLJ11149,RIFK - Gene ID
- 55312
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