RFP/DsRed antibody

Antigen: RFP/DsRed

Clonality: Polyclonal

Host: Rabbit

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN132020

Additional Information

Immunogen: This antibody was purified by protein A Sepharose from rabbit polyclonal antibody raised against the RFP (DsRed) fluorescent protein.

Format: Liquid

Description: Red Fluorescent Protein (RFP)-Tag fusion protein expression is commonly used in many laboratories for monitoring gene expression and protein localization in living cells. This antibody for RFP-Tag is a useful tool for detecting RFP-fusion protein expression.

Specificity: This antibody reacts with RFP-tagged fusion proteins on western blotting and immunocytochemistry. Recognizes mDsRed and mCherry.

Application Details

Application Notes: Western blotting : 1:1,000 for chemiluminescence detection. Immunocytochemistry : 1:500

Buffer: Supplied in 100 µL PBS containing 0.3 M NaCl, 10 mM phosphate buffer, pH 8.0 and 0.09% sodium azide. * Azide may react with copper or lead in plumbing systems to form explosive metal azides. Therefore, always flush drains thoroughly with plenty of water w

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Western Blotting: analysis of DsRed fusion protein (1), mCherry (2) and mRFP1 (3)

Publications

Publications: Matsuura, Wada, Nagai et al.: "Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle." in: The Journal of cell biology, Vol. 167, Issue 2, pp. 351-63, 2004 (PubMed).

Tsuboi, Fukuda: "The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex." in: Molecular biology of the cell, Vol. 17, Issue 5, pp. 2101-12, 2006 (PubMed).

Toyooka, Moriyasu, Goto et al.: "Protein aggregates are transported to vacuoles by a macroautophagic mechanism in nutrient-starved plant cells." in: Autophagy, Vol. 2, Issue 2, pp. 96-106, 2006 (PubMed).

Tsuboi, Kanno, Fukuda: "The polybasic sequence in the C2B domain of rabphilin is required for the vesicle docking step in PC12 cells." in: Journal of neurochemistry, Vol. 100, Issue 3, pp. 770-9, 2007 (PubMed).