Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody

Antigen: Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)

Synonyms: LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1

Clonality: Monoclonal (VIT200)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Catalog no.: ABIN132120

Additional Information

Alternative name: CD45

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: VIT200

Specificity: The CD45 molecule is typically expressed at high levels on all hematopoietic cells. CD45 is a major component of the glycocalix of these cells and can be expressed in different isoforms. Antibody VIT200 recognizes a pan CD45 epitope, which is expressed on all hematopoietic cells.The CD45 mAb (clone VIT200) recognizes a pan CD45 epitope.

Application Details

Application Notes: The VIT200 antibody permits the identification and enumeration of human leukocytes using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of VIT200 mAb is determined by staining welldefined blood samples from representative donors with serialfold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ?l of leukocytes containing 107cells/ml are stained with 20 ?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Concentration: 0,1 mg/ml

Purification: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody

Publications

Publications: Schraven, Roux, Hutmacher et al.: "Triggering of the alternative pathway of human T cell activation involves members of the T 200 family of glycoproteins." in: European journal of immunology, Vol. 19, Issue 2, pp. 397-403, 1989 (PubMed).

Thomas: "The leukocyte common antigen family." in: Annual review of immunology, Vol. 7, pp. 339-69, 1989 (PubMed).

Sugita, Majdic, Stockinger et al.: "Induction of human complement activation without cytolysis by mouse monoclonal antibodies to human leukocyte antigens." in: Transplantation, Vol. 43, Issue 4, pp. 570-4, 1987 (PubMed).

Hale, Cobbold, Waldmann et al.: "Isolation of low-frequency class-switch variants from rat hybrid myelomas." in: Journal of immunological methods, Vol. 103, Issue 1, pp. 59-67, 1987 (PubMed).

Battifora, Trowbridge: "A monoclonal antibody useful for the differential diagnosis between malignant lymphoma and nonhematopoietic neoplasms." in: Cancer, Vol. 51, Issue 5, pp. 816-21, 1983 (PubMed).

Omary, Trowbridge, Battifora: "Human homologue of murine T200 glycoprotein." in: The Journal of experimental medicine, Vol. 152, Issue 4, pp. 842-52, 1980 (PubMed).

Dalchau, Kirkley, Fabre: "Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat." in: European journal of immunology, Vol. 10, Issue 10, pp. 737-44, 1981 (PubMed).

Nicholson, Hubbard, Jones: "Use of CD45 fluorescence and side-scatter characteristics for gating lymphocytes when using the whole blood lysis procedure and flow cytometry." in: Cytometry, Vol. 26, Issue 1, pp. 16-21, 1996 (PubMed).

Sun, Sangaline, Ryder et al.: "Gating strategy for immunophenotyping of leukemia and lymphoma." in: American journal of clinical pathology, Vol. 108, Issue 2, pp. 152-7, 1997 (PubMed).

Brocklebank, Sparrow: "Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy." in: Cytometry, Vol. 46, Issue 4, pp. 254-61, 2001 (PubMed).

Jing, Yuan, Feng et al.: "[Endovascular exclusion of juxtrarenal abdominal aortic aneurysm with one-piece customized fenestrated endovascular stent-graft]" in: Zhonghua wai ke za zhi [Chinese journal of surgery], Vol. 45, Issue 23, pp. 1596-9, 2008 (PubMed).