CD14 Molecule (CD14) antibody

Antigen: CD14 Molecule (CD14)

Synonyms: CD14

Clonality: Monoclonal (MEM18)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN132122

Additional Information

Alternative name: CD14

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MEM18

Specificity: CD14 is a GPI-anchored molecule expressed by virtually all human monocytes and macrophages and

Application Details

Application Notes: The MEM18 antibody permits the identification and enumeration of leukocytes using flow cytometry. MEM18 has been also used for functional studies since this antibody blocks the interaction of LPS with CD14 on monocytes. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of MEM18 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ?l of leukocytes containing 107cells/ml are stained with 20 ?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Purification: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-CD14 Molecule (CD14) antibody

Publications

Publications: Johnson, Greb, Goyert et al.: "Midtrimester diagnosis and anomalies in the dup(22q) syndrome: correlation of aneuploidy with low maternal serum alpha-fetoprotein and oligohydramnios." in: American journal of medical genetics, Vol. 36, Issue 1, pp. 94-6, 1990 (PubMed).

Goyert, Ferrero, Rettig et al.: "The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors." in: Science (New York, N.Y.), Vol. 239, Issue 4839, pp. 497-500, 1988 (PubMed).

Goyert, Ferrero, Seremetis et al.: "Biochemistry and expression of myelomonocytic antigens." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 12, pp. 3909-14, 1987 (PubMed).

Ugolini, Nunez, Smith et al.: "Initial characterization of monoclonal antibodies against human monocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, Issue 11, pp. 6764-8, 1981 (PubMed).

Knapp: "Monoclonal antibodies against differentiation antigens of myelopoiesis." in: Blut, Vol. 45, Issue 5, pp. 301-8, 1983 (PubMed).

Juan, Kelley, Johnson et al.: "Soluble CD14 truncated at amino acid 152 binds lipopolysaccharide (LPS) and enables cellular response to LPS." in: The Journal of biological chemistry, Vol. 270, Issue 3, pp. 1382-7, 1995 (PubMed).

Juan, Hailman, Kelley et al.: "Identification of a lipopolysaccharide binding domain in CD14 between amino acids 57 and 64." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5219-24, 1995 (PubMed).

Yoshimura, Lien, Ingalls et al.: "Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 1, pp. 1-5, 1999 (PubMed).

Means, Lien, Yoshimura et al.: "The CD14 ligands lipoarabinomannan and lipopolysaccharide differ in their requirement for Toll-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6748-55, 2000 (PubMed).

Tapping, Akashi, Miyake et al.: "Toll-like receptor 4, but not toll-like receptor 2, is a signaling receptor for Escherichia and Salmonella lipopolysaccharides." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 10, pp. 5780-7, 2000 (PubMed).

Perera, Mayadas, Takeuchi et al.: "CD11b/CD18 acts in concert with CD14 and Toll-like receptor (TLR) 4 to elicit full lipopolysaccharide and taxol-inducible gene expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 574-81, 2001 (PubMed).

Beutler: "TLR4 as the mammalian endotoxin sensor." in: Current topics in microbiology and immunology, Vol. 270, pp. 109-20, 2002 (PubMed).

Franz, Knapp: "Staged Endovascular Repair of Bilateral Internal Iliac Artery Aneurysms." in: Annals of vascular surgery, 2008 (PubMed).