Lysozyme (LYZ) antibody (FITC)

Antigen: Lysozyme (LYZ)

Synonyms: LZM, lysozyme, lysD, DmelCG9118, CG9118, lysP, DmelCG9116, CG9116, LYZ, MGC136734, zgc:136734, BmLys, LZ, LYS, MGC137103, LYSOZYME, LYZS

Clonality: Monoclonal (LZ-1)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Catalog no.: ABIN132131

Additional Information

Alternative name: Lysozym

Format: Purified

Isotype: IgG1

Clone: LZ-1

Specificity: Lysozyme (LZ) is a cationic antimicrobial peptide of 14 kDa. Lysozyme is stored in primary but predominantly in specific (secondary) granules of neutrophils. It cleaves peptidoglycan constituents of the bacterial cell wall and can bind LPS. The epitope recognized by antibody LZ-2 is expressed by virtually all myeloid cells including normal and malignant granulocytes and monocytes. In normal myelopoiesis LZ can first be detected at the myeloblast stage where it appears somewhat later than MPO expression. The anti-Lysozyme Antibody (clone LZ-2) reacts with intracellular human lysozyme/muramidase expressed by virtually all myelomonocytic cells, macrophages and their precursors.

Application Details

Application Notes: The LZ-2 antibody permits the identification and enumeration of human myelomonocytic cells using flow cytometry. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: Biological fluids (blood, bone marrow, and others) must be collected under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of LZ-2 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAbconcentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ?l of leukocytes containing 107cells/ml are stained with 20 ?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Purification: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-Lysozyme (LYZ) antibody (FITC)

Publications

Publications: Srivastava, Rado, Bauerle et al.: "Regulation of human bone marrow lactoferrin and myeloperoxidase gene expression by tumor necrosis factor-alpha." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 3, pp. 1014-9, 1991 (PubMed).

Catovsky, Matutes, Buccheri et al.: "A classification of acute leukaemia for the 1990s." in: Annals of hematology, Vol. 62, Issue 1, pp. 16-21, 1991 (PubMed).

Cramer, Pryzwansky, Villeval et al.: "Ultrastructural localization of lactoferrin and myeloperoxidase in human neutrophils by immunogold." in: Blood, Vol. 65, Issue 2, pp. 423-32, 1985 (PubMed).

Rado, Wei, Benz: "Isolation of lactoferrin cDNA from a human myeloid library and expression of mRNA during normal and leukemic myelopoiesis." in: Blood, Vol. 70, Issue 4, pp. 989-93, 1987 (PubMed).

Rado, Bollekens, St Laurent et al.: "Lactoferrin biosynthesis during granulocytopoiesis." in: Blood, Vol. 64, Issue 5, pp. 1103-9, 1984 (PubMed).

He, Furmanski: "Sequence specificity and transcriptional activation in the binding of lactoferrin to DNA." in: Nature, Vol. 373, Issue 6516, pp. 721-4, 1995 (PubMed).

Knapp, Majdic, Strobl: "Flow cytometric analysis of intracellular myeloperoxidase and lactoferrin in leukemia diagnosis." in: Recent results in cancer research. Fortschritte der Krebsforschung. Progrès dans les recherches sur le cancer, Vol. 131, pp. 31-40, 1993 (PubMed).

Groeneveld, te Marvelde, van den Beemd et al.: "Flow cytometric detection of intracellular antigens for immunophenotyping of normal and malignant leukocytes." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 10, Issue 8, pp. 1383-9, 1996 (PubMed).

Gullberg, Andersson, Garwicz et al.: "Biosynthesis, processing and sorting of neutrophil proteins: insight into neutrophil granule development." in: European journal of haematology, Vol. 58, Issue 3, pp. 137-53, 1997 (PubMed).

Oehler, Majdic, Pickl et al.: "Neutrophil granulocyte-committed cells can be driven to acquire dendritic cell characteristics." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1019-28, 1998 (PubMed).

Konikova, Glasova, Kusenda et al.: "Intracellular markers in acute myeloid leukemia diagnosis." in: Neoplasma, Vol. 45, Issue 5, pp. 282-91, 1999 (PubMed).

Cowland, Borregaard: "The individual regulation of granule protein mRNA levels during neutrophil maturation explains the heterogeneity of neutrophil granules." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 989-95, 2000 (PubMed).

Braylan, Orfao, Borowitz et al.: "Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting." in: Cytometry, Vol. 46, Issue 1, pp. 23-7, 2001 (PubMed).

Teng, Gladwell, Beard et al.: "Lactoferrin gene expression is estrogen responsive in human and rhesus monkey endometrium." in: Molecular human reproduction, Vol. 8, Issue 1, pp. 58-67, 2001 (PubMed).

Paietta: "How to optimize multiparameter flow cytometry for leukaemia/lymphoma diagnosis." in: Best practice & research. Clinical haematology, Vol. 16, Issue 4, pp. 671-83, 2003 (PubMed).

Strobl, Knapp: "Myeloid cell-associated lysosomal proteins as flow cytometry markers for leukocyte lineage classification." in: Journal of biological regulators and homeostatic agents, Vol. 18, Issue 3-4, pp. 335-9, 2005 (PubMed).