Interferon gamma (IFNG) antibody (FITC)

Antigen: Interferon gamma (IFNG)

Synonyms: IFG, IFI, Ifg, IFN-g, IFNG2, IFNG, IFN-gamma

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Catalog no.: ABIN132136

Additional Information

Alternative name: Interferon gamma

Format: Purified

Isotype: IgG1

Specificity: Interferon ? is a type-2 interferon that is produced mainly by T-cells and NK-cell upon cellular activation. Human CD8 T-cells, isolated from individuals after viral infection or vaccination, release IFN-? upon exposure to the corresponding viral antigens in vitro. The anti-Interferon ? Antibody (clone GZ4) reacts with intracellular human interferon ? expressed by activated T-cells and NK cells.

Application Details

Application Notes: The GZ4 antibody permits the analysis of activated human T-cells and T-cell clones as well as NK cells using flow cytometry. To allow intracellular accumulation of IFN ? (prevent secretion), cells of interest can be pretreated with e.g. Brefeldin A (2 ?g/ml) for 18 hours before analyses. Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us. Samples: under sterile conditions. Anticoagulation with EDTA or heparin is recommended. The samples should be stored at room temperature until used. For optimal results, samples should be processed and analyzed within 24 hours. Samples with high numbers of non-viable cells might cause false results, such cases require determination of cell viability with e.g. propidium iodide. All biological samples have to be handled with caution. Always consider them as potentially infective. Use appropriate precautions such as gloves, lab-coat, etc. Sensitivity: The sensitivity of GZ4 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAbconcentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ?l of leukocytes containing 107cells/ml are stained with 20 ?l mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.

Purification: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-Interferon gamma (IFNG) antibody (FITC)

Publications

Publications: Sander, Andersson, Andersson: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, 1991 (PubMed).

Rubartelli, Cozzolino, Talio et al.: "A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence." in: The EMBO journal, Vol. 9, Issue 5, pp. 1503-10, 1990 (PubMed).

Celis, Miller, Wiktor et al.: "Isolation and characterization of human T cell lines and clones reactive to rabies virus: antigen specificity and production of interferon-gamma." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 2, pp. 692-7, 1986 (PubMed).

Yip, Barrowclough, Urban et al.: "Purification of two subspecies of human gamma (immune) interferon." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 6, pp. 1820-4, 1982 (PubMed).

Rinderknecht, OConnor, Rodriguez: "Natural human interferon-gamma. Complete amino acid sequence and determination of sites of glycosylation." in: The Journal of biological chemistry, Vol. 259, Issue 11, pp. 6790-7, 1984 (PubMed).

Funane, Yamada, Shiraiwa et al.: "Aggregated form of dextransucrases from Leuconostoc mesenteroides NRRL B-512F and its constitutive mutant." in: Bioscience, biotechnology, and biochemistry, Vol. 59, Issue 5, pp. 776-80, 1995 (PubMed).

Elson, Nutman, Metcalfe et al.: "Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 9, pp. 4294-301, 1995 (PubMed).

Jung, Schauer, Heusser et al.: "Detection of intracellular cytokines by flow cytometry." in: Journal of immunological methods, Vol. 159, Issue 1-2, pp. 197-207, 1993 (PubMed).

Waclavicek, Majdic, Stulnig et al.: "CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 9, pp. 4671-8, 1998 (PubMed).

Schuerwegh, Stevens, Bridts et al.: "Evaluation of monensin and brefeldin A for flow cytometric determination of interleukin-1 beta, interleukin-6, and tumor necrosis factor-alpha in monocytes." in: Cytometry, Vol. 46, Issue 3, pp. 172-6, 2001 (PubMed).