T Cell Receptor (TCR) alpha/beta (alpha chain) antibody (PE)

Antigen: T Cell Receptor (TCR) alpha/beta (alpha chain)

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN132137

Additional Information

Alternative name: TCR alpha/beta

Format: Purified

Isotype: IgG1

Specificity: T-lymphocytes recognize foreign antigens via their clonotypic T-cell receptor (TCR). The TCR consists of two disulfide-linked TCR chains, which are embedded in a sheath of CD3 molecules, commonly referred as the TCR/CD3 complex. Among TCR heterodimers two forms can be distinguished: ?/? TCRs and ?/? TCRs. T-lymphocytes expressing the ?/? TCR make up the majority of T-cells in peripheral blood and bone marrow and can be distinguished from ?/? Tlymphocytes expressing the ?/? TCR. In normal T-cell development ?/? TCR can first be detected in the cortical thymocyte stage. A variety of studies have demonstrated the usefulness and reliability of anti-TCR mAbs for the classification of acute T-cell leukemias (T-ALL). In T-ALL surface ?/? TCR expression is characteristic for the T-ALL IVa subtype according to the EGIL classification. Antibody BMA 031 reacts with a constant determinant expressed on the surface of all ?/? TCR expressing T-lymphocytes.

Application Details

Application Notes: MAb BMA 031 binds to T-lymphocytes expressing the ?/? TCR/CD3 complex. The ?/? T-cell receptor (TCR) is the specific antigen receptor of the majority of human T-lymphocytes. It is expressed on thymocytes and peripheral T cells, which don

Purification: Chromatography

Buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage: For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

anti-T Cell Receptor (TCR) alpha/beta (alpha chain) antibody (PE)

Publications

Publications: Schwinzer, Schlitt, Wonigeit: "Monoclonal antibodies to common epitopes of the human alpha/beta T-cell receptor preferentially activate CD45RA+ T-cells." in: Cellular immunology, Vol. 140, Issue 1, pp. 31-41, 1992 (PubMed).

Borst, van Dongen, de Vries et al.: "BMA031, a monoclonal antibody suited to identify the T-cell receptor alpha beta/CD3 complex on viable human T lymphocytes in normal and disease states." in: Human immunology, Vol. 29, Issue 3, pp. 175-88, 1991 (PubMed).

Janssen, Wesselborg, Heckl-Ostreicher et al.: "T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 1, pp. 35-9, 1991 (PubMed).

Kabelitz, Bender, Schondelmaier et al.: "Human cytotoxic lymphocytes. V. Frequency and specificity of gamma delta+ cytotoxic lymphocyte precursors activated by allogeneic or autologous stimulator cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 9, pp. 2827-32, 1990 (PubMed).

Miossec, Faure, Ferradini et al.: "Further analysis of the T cell receptor gamma/delta+ peripheral lymphocyte subset. The V delta 1 gene segment is expressed with either C alpha or C delta." in: The Journal of experimental medicine, Vol. 171, Issue 4, pp. 1171-88, 1990 (PubMed).

Nashan, Wonigeit, Schwinzer et al.: "Fine specificity of a panel of antibodies against the TCR/CD3 complex." in: Transplantation proceedings, Vol. 19, Issue 5, pp. 4270-2, 1987 (PubMed).

Bene, Castoldi, Knapp et al.: "Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL)." in: Leukemia : official journal of the Leukemia Society of America, Leukemia Research Fund, U.K, Vol. 9, Issue 10, pp. 1783-6, 1995 (PubMed).

Peyrat, Davodeau, Houde et al.: "Repertoire analysis of human peripheral blood lymphocytes using a human V delta 3 region-specific monoclonal antibody. Characterization of dual T cell receptor (TCR) delta-chain expressors and alpha beta T cells expressing V delta 3J alpha C alpha-encoded" in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 6, pp. 3060-7, 1995 (PubMed).

Davodeau, Peyrat, Romagne et al.: "Dual T cell receptor beta chain expression on human T lymphocytes." in: The Journal of experimental medicine, Vol. 181, Issue 4, pp. 1391-8, 1995 (PubMed).

Thibault, Bardos: "Compared TCR and CD3 epsilon expression on alpha beta and gamma delta T cells. Evidence for the association of two TCR heterodimers with three CD3 epsilon chains in the TCR/CD3 complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3814-20, 1995 (PubMed).

Knight, Kurrle, McClain et al.: "Clinical evaluation of induction immunosuppression with a murine IgG2b monoclonal antibody (BMA 031) directed toward the human alpha/beta-T cell receptor." in: Transplantation, Vol. 57, Issue 11, pp. 1581-8, 1994 (PubMed).