BCL2-Associated X Protein (BAX) antibody

Antigen: BCL2-Associated X Protein (BAX)

Synonyms: BCL2L4, bax, fj16e01, wu:fc50b10, wu:fj16e01, BAX, bax-A, xBax, xlbax, MGC76049, BAX-ALPHA

Clonality: Monoclonal (5B7)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Catalog no.: ABIN135026

Additional Information

Alternative name: Bax

Immunogen: KLH-conjugated peptide corresponding to amino acids 3-16 near the N-terminus of murine Bax 1 Ig

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 5B7

Description: Mouse Bax (Mr 21 kDa) Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program.4-13 Mab 5B7 is specific for mouse Bax.

Application Details

Application Notes: WORKING DILUTIONS Western Blotting:Purified antibody 1 ug/mL Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The products should be stored at 2-8C. Each product is stable for the period shown on the label if stored as directed.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Cohen: "Programmed cell death in the immune system." in: Advances in immunology, Vol. 50, pp. 55-85, 1991 (PubMed).

Rajah, Bhala, Nunn et al.: "7S nerve growth factor is an insulin-like growth factor-binding protein protease." in: Endocrinology, Vol. 137, Issue 7, pp. 2676-82, 1996 (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).