CD8a antibody (FITC)

Antigen: CD8a

Clonality: Monoclonal (53-6-7)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN135120

Additional Information

Alternative name: CD8a/Ly-2

Isotype: IgG2a

Clone: 53-6-7

Description: Chain of the murine CD8 heterodimer, Mr 32-34 kDa In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified antibody and Fluorescein and Biotin (2 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells), R-phycoerythrin-Texas Red (0.2 ug/10^6 cells), Allophycocyanin (0.2 ug/10^6 cells), Spectral RedTM (0.2 ug/10^6 cells), Cyanine 5 (0.2 ug/10^6 cells), R-phycoerythrin-Cyanine 5.5 (0.2 ug/10^6 cells), R-phycoerythrin-Cyanine 7 (0.2 ug/10^6 cells), Allophycocyanin-Cyanine 5.5 (0.2 ug/10^6 cells), Allophycocyanin-Cyanine 7 (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY5.5), allophycocyanin-Cyanine 5.5 conjugate (APC-CY5.5), allophycocyanin-Cyanine 7 (APC-CY7) and R-phycoerythrin-Texas Red (R-PE-TXRD) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20C. The Cyanine 5 (CY5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Images

anti-CD8a antibody (FITC)

Publications

Publications: Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, 1991 (PubMed).

Lefrancois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992 (PubMed).

Takahasi, Kondou, Watanabe et al.: "[Investigation of etching and bonding on the inner surface of free enamel. SEM observation and thermal cycling test for pigment invasion]" in: Shika gakuho. Dental science reports, Vol. 89, Issue 3, pp. 663-8, 1990 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Ledbetter, Seaman: "The Lyt-2, Lyt-3 macromolecules: structural and functional studies." in: Immunological reviews, Vol. 68, pp. 197-218, 1983 (PubMed).