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CD40 Ligand antibody (CD40LG) (PE)

Details for Product anti-CD40LG Antibody No. ABIN135218, Supplier: Log in to see
Antigen
  • CD154
  • cd40l
  • CD40L
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TNFSF5
  • TRAP
  • gp39
  • hCD40L
  • CD40-L
  • Cd40l
  • Ly-62
  • Ly62
  • Tnfsf5
Alternatives
anti-Mouse (Murine) CD40 Ligand antibody for Flow Cytometry
Reactivity
Mouse (Murine)
366
151
40
15
14
11
7
4
3
3
3
2
2
2
2
Host
Hamster
219
123
88
26
21
19
Clonality (Clone)
Monoclonal ()
Conjugate
This CD40 Ligand antibody is conjugated to PE
72
54
35
8
6
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
270
190
135
53
51
49
40
31
22
15
11
7
6
6
6
5
4
3
3
3
2
2
1
1
1
1
Supplier
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Immunogen Activated mouse Th1 clone D1.6 1
Clone MR1
Isotype IgG
Characteristics The antibody detects: CD154 (Mouse CD40 ligand/gp39), Mr 39 kDa
The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function. Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens, yet does not alter the immune response to TI antigens.
Alternative Name CD154/CD40L (CD40LG Antibody Abstract)
Background CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporin-sensitive.
Pathways NF-kappaB Signaling
Application Notes Working Dilution:
FACS: 02 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo and in vitro blockage of CD154 function

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.1 mg in 1.0 mL of PBS / NaN3 and a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Roy, Waldschmidt, Aruffo et al.: "The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2497-510, 1993 (PubMed).

Foy, Shepherd, Durie et al.: "In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity. II. Prolonged suppression of the humoral immune response by an antibody to the ligand for CD40, gp39." in: The Journal of experimental medicine, Vol. 178, Issue 5, pp. 1567-75, 1993 (PubMed).

Aruffo, Farrington, Hollenbaugh et al.: "The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome." in: Cell, Vol. 72, Issue 2, pp. 291-300, 1993 (PubMed).

Foy, Page, Waldschmidt et al.: "An essential role for gp39, the ligand for CD40, in thymic selection." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, 1995 (PubMed).

Hollenbaugh, Grosmaire, Kullas et al.: "The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity." in: The EMBO journal, Vol. 11, Issue 12, pp. 4313-21, 1992 (PubMed).

Noelle, Roy, Shepherd et al.: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992 (PubMed).

Armitage, Fanslow, Strockbine et al.: "Molecular and biological characterization of a murine ligand for CD40." in: Nature, Vol. 357, Issue 6373, pp. 80-2, 1992 (PubMed).