Selectin L (SELL) antibody

Antigen: Selectin L (SELL)

Synonyms: TQ1, LAM1, LNHR, LSEL, CD62L, LAM-1, LYAM1, Leu-8, PLNHR, hLHRc, LECAM1, Lyam-1, Lnhr, Ly-22, Lyam1, Ly-m22, LECAM-1, AI528707, L-selectin

Clonality: Monoclonal (MEL-14)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Catalog no.: ABIN135249

Additional Information

Alternative name: CD62L/L-selectin

Immunogen: C3H/eb cloned mouse B lymphoma 38C-13 1

Isotype: IgG2a

Clone: MEL-14

Description: CD62L/L-selectin, Mr 90 kDa CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains.1, 2 It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes.1, 2 In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils.3-7 This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation.1, 2, 7-9 Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.10-13 The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.1,

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Fluorescein (2 ug/10^6 cells), Biotin (2 ug/10^6 cells), R-phycoerythrin (0.1 ug/10^6 cells), Allophycocyanin and Allophycocyanin-Cyanine 7 (0.1 ug/10^6 cells), Spectral RedTM (0.1 ug/10^6 cells), R-phycoerythrin-Cyanine 7 (0.1 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.

Purification: Ion exchange column

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral RedTM (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CYTM7), and allophycocyanin-Cyanine 7 (APC-CYTM7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20C. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983 (PubMed).

Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, 1994 (PubMed).