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L-Selectin antibody (Selectin L)

Details for Product anti-SELL Antibody No. ABIN135253, Supplier: Log in to see
Antigen
  • CD62L
  • LAM1
  • LECAM1
  • LEU8
  • LNHR
  • LSEL
  • LYAM1
  • PLNHR
  • TQ1
  • A.11
  • L-selectin
  • LECAM-1
  • SELE
  • AI528707
  • Lnhr
  • Ly-22
  • Ly-m22
  • Lyam-1
  • Lyam1
Reactivity
Mouse (Murine)
355
87
73
23
10
1
1
1
Host
Rat
256
182
85
15
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This L-Selectin antibody is un-conjugated
89
76
34
31
13
8
7
7
6
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
426
138
120
115
70
45
40
21
14
13
11
9
6
5
5
4
3
2
2
2
2
2
2
2
2
1
1
Supplier
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Immunogen C3H/eb cloned mouse B lymphoma 38C-13 1
Clone MEL-14
Isotype IgG2a
Characteristics The antibody detects: CD62L/L-selectin, Mr 90 kDa
The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.
Purification boric acid precipitation
Endotoxin Level Low Endotoxin
Alternative Name CD62L/L-selectin (SELL Antibody Abstract)
Background CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.
Pathways
Application Notes Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo and in vitro blocking of adhesion, Cytotoxicity studies

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage -20 °C
Storage Comment Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
Background publications Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, 1994 (PubMed).

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983 (PubMed).