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CD72.1 antibody (Biotin)

Details for Product No. ABIN135266, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine)
21
Host
Mouse
21
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
6
6
5
Application
Flow Cytometry (FACS)
21
Supplier
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Immunogen DBA/2 mouse spleen cells 1
Clone 10-1-D2
Isotype IgG2b
Characteristics The antibody detects: Mouse CD72.1/Lyb-2.1, 45 kDa Murine
However, 10-1.D.2 can itself trigger a positive signal by binding CD72.
Purification Ion exchange
Background CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells. The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing.
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Working Dilution:
1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vitro blocking and proliferation assays

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Luo, Van de Velde, von Hoegen et al.: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, 1992 (PubMed).

Beavil, Edmeades, Gould et al.: "Alpha-helical coiled-coil stalks in the low-affinity receptor for IgE (Fc epsilon RII/CD23) and related C-type lectins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 753-7, 1992 (PubMed).