Ly-6C antibody

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Antigen
  • AA682074
  • AA959465
  • Ly-6C
  • Ly-6C1
  • Ly6c
  • lymphocyte antigen 6 complex, locus C1
  • Ly6c1
Reactivity
Mouse (Murine)
97
Host
Rat
96
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
14
14
10
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
92
25
23
9
4
4
4
2
2
1
1
1
1
1
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Clone HK1-4
Isotype IgG2c
Characteristics The antibody detects: Murine Ly-6C, Mr 18 kDa
Purification Purified
Background Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vitro activation of T lymphocytes

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Product cited in: Chen, Grimbaldeston, Tsai, Weissman, Galli: "Identification of mast cell progenitors in adult mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 32, pp. 11408-13, 2005 (PubMed).

Background publications Lancki, Fields, Qian, Fitch: "Induction of lytic pathways in T cell clones derived from wild-type or protein tyrosine kinase Fyn mutant mice." in: Immunological reviews, Vol. 146, pp. 117-44, 1996 (PubMed).

Herold, Montag, Meyer, Wojcikowski, Fitch: "Expression of Ly-6C by T lymphocytes of NOD mice after CD3-complex stimulation. Identification of activated cells during insulitis of prediabetic mice." in: Diabetes, Vol. 39, Issue 7, pp. 815-20, 1990 (PubMed).