CD3 epsilon antibody

Catalog No. ABIN135464

Product Details anti-CD3 epsilon Antibody

Target: CD3 epsilon (CD3E)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Porcine CD3, Mr 23 kDa

Characteristics: Mouse Anti-Porcine CD3e-UNLB

Purification: Purified

Immunogen: Purified CD3 molecules from porcine thymus

Clone: PPT3

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Sep - Reported in literature , Stim - Reported in literature , Cyto - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro activation of T cells

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added

Preservative: Without preservative

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD3 epsilon antibody (ABIN135464)

Lei, Ryu, Wen, Twitchell, Bui, Ramesh, Weiss, Li, Samuel, Clark-Deener, Jiang, Lee, Yuan: "Increased and prolonged human norovirus infection in RAG2/IL2RG deficient gnotobiotic pigs with severe combined immunodeficiency." in: Scientific reports, Vol. 6, pp. 25222, (2018) (PubMed).

Erneholm, Lorenzen, Bøje, Olsen, Andersen, Cassidy, Follmann, Jensen, Agerholm: "Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D." in: BMC veterinary research, Vol. 12, Issue 1, pp. 200, (2017) (PubMed).

Twitchell, Tin, Wen, Zhang, Becker-Dreps, Azcarate-Peril, Vilchez, Li, Ramesh, Weiss, Lei, Bui, Yang, Schultz-Cherry, Yuan: "Modeling human enteric dysbiosis and rotavirus immunity in gnotobiotic pigs." in: Gut pathogens, Vol. 8, pp. 51, (2016) (PubMed).

Wen, Bui, Li, Liu, Li, Kocher, Yuan: "Characterization of immune modulating functions of γδ T cell subsets in a gnotobiotic pig model of human rotavirus infection." in: Comparative immunology, microbiology and infectious diseases, (2012) (PubMed).

Isling, Aalbaek, Schrøder, Leifsson: "Pyelonephritis in slaughter pigs and sows: morphological characterization and aspects of pathogenesis and aetiology." in: Acta veterinaria Scandinavica, Vol. 52, pp. 48, (2010) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e (CD3E Products)

Synonyms: cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody

Background: Porcine CD3 is a member of the T-cell receptor-associated CD3 complex. It is found on a subpopulation of thymocytes and on all pig T lymphocytes. MAb PPT3 is mitogenic when presented to peripheral blood mononuclear cells in immobilized form.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway