Ezrin antibody (pTyr353)

Catalog No. ABIN1355833

Product Details anti-Ezrin Antibody

Target: Ezrin (EZR)

Binding Specificity: pTyr353

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

Immunogen: Peptide sequence around phosphorylation site of pTyr353 (Q-D-Y (p) -E-E) derived from Human EZRIN. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100  

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Ezrin antibody (ABIN1355833)

Oda, Aishima, Morimatsu, Hayashi, Shindo, Fujino, Mizuuchi, Hattori, Tanaka, Oda: "Differential ezrin and phosphorylated ezrin expression profiles between pancreatic intraepithelial neoplasia, intraductal papillary mucinous neoplasm, and invasive ductal carcinoma of the pancreas." in: Human pathology, Vol. 44, Issue 8, pp. 1487-98, (2013) (PubMed).

Morimatsu, Aishima, Kayashima, Hayashi, Nakata, Oda, Taguchi, Tsuneyoshi, Tanaka, Oda: "Liver-intestine cadherin expression is associated with intestinal differentiation and carcinogenesis in intraductal papillary mucinous neoplasm." in: Pathobiology : journal of immunopathology, molecular and cellular biology, Vol. 79, Issue 2, pp. 107-14, (2012) (PubMed).

Cui, Li, Zhang, Han: "Expression of Ezrin and phosphorylated Ezrin (pEzrin) in pancreatic ductal adenocarcinoma." in: Cancer investigation, Vol. 28, Issue 3, pp. 242-7, (2010) (PubMed).

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin (EZR Products)

Synonyms: CVIL antibody, CVL antibody, VIL2 antibody, ezr antibody, vil2 antibody, wu:fc05c03 antibody, wu:fj31d06 antibody, cvil antibody, cvl antibody, Vil2 antibody, AW146364 antibody, R75297 antibody, p81 antibody, ezr.S antibody, ezrin antibody, ezrin b antibody, ezrin L homeolog antibody, EZR antibody, ezrb antibody, ezr antibody, Ezr antibody, ezr.L antibody

Background: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Molecular Weight: 81 kDa

NCBI Accession: NP_001104

UniProt: P15311

Pathways: Maintenance of Protein Location