Histone 3 antibody (pThr11)
-
- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
-
Binding Specificity
- pThr11
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Histone 3 antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Specificity
- The antibody detects endogenous level of Histone H3 only when phosphorylated at threonine 11.
- Immunogen
- Peptide sequence around phosphorylation site of pThr11 (K-S-T (p) -G-G) derived from Human Histone H3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Top Product
- Discover our top product H3 Primary Antibody
-
-
- Application Notes
- Western blotting: 1:500-1:1000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Background
- Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- Molecular Weight
- 17 kDa
- NCBI Accession
- NP_066403, NP_002098
- UniProt
- P84243
-