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JAK2 antibody (pTyr221)

JAK2 Reactivity: Human, Rat, Mouse IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1355856
  • Target See all JAK2 Antibodies
    JAK2 (Janus Kinase 2 (JAK2))
    Binding Specificity
    • 37
    • 34
    • 16
    • 16
    • 13
    • 12
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr221
    Reactivity
    • 178
    • 101
    • 97
    • 18
    • 18
    • 3
    • 2
    • 2
    • 1
    Human, Rat, Mouse
    Host
    • 180
    • 6
    • 2
    Rabbit
    Clonality
    • 164
    • 24
    Polyclonal
    Conjugate
    • 93
    • 16
    • 10
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This JAK2 antibody is un-conjugated
    Application
    • 136
    • 86
    • 62
    • 61
    • 29
    • 27
    • 26
    • 25
    • 17
    • 13
    • 10
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Specificity
    The antibody detects endogenous level of JAK2 only when phosphorylated at tyrosine 221.
    Immunogen
    Peptide sequence around phosphorylation site of pTyr221 (Q-D-Y (p) -H-I) derived from Human JAK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Top Product
    Discover our top product JAK2 Primary Antibody
  • Application Notes
    Immunohistochemistry: 1:50-1:100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C
  • Target
    JAK2 (Janus Kinase 2 (JAK2))
    Alternative Name
    JAK2 (JAK2 Products)
    Synonyms
    JAK2bCA antibody, jak2 antibody, JTK10 antibody, THCYT3 antibody, AI504024 antibody, C81284 antibody, Fd17 antibody, JAK-2 antibody, Janus kinase 2b antibody, Janus kinase 2 antibody, jak2b antibody, JAK2 antibody, Jak2 antibody
    Background
     Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO), or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Molecular Weight
    125 kDa
    NCBI Accession
    NM_004972, NP_004963
    UniProt
    O60674
    Pathways
    JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response
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