Bu-1 antibody (FITC)

Antigen: Bu-1

Clonality: Monoclonal (AV20)

Host: Mouse

Reactivity: Chicken, Bird (Avian)

Conjugate: FITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN135595

Additional Information

Immunogen: Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens1

Format: Purified

Isotype: IgG1

Clone: AV20

Description: Chicken Bu-1 (chB6), Mr 70-75 kDa Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.1-2 MAb AV20 does not cross react with turkey cells when analyzed by immunofluorescent staining and flow cytometry.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (1 ug/10^6 cells), Biotin (1 ug/10^6 cells), R-phycoerythrin (0.2 ug/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Publications: Tregaskes, Bumstead, Davison et al.: "Chicken B-cell marker chB6 (Bu-1) is a highly glycosylated protein of unique structure." in: Immunogenetics, Vol. 44, Issue 3, pp. 212-7, 1996 (PubMed).