CD11b antibody (Integrin alpha M) (PE)

Details for Product anti-ITGAM Antibody No. ABIN135738, Supplier: Log in to see
Antigen
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha M
  • integrin, alpha M
  • ITGAM
  • Itgam
Alternatives
anti-Human CD11b antibody for Biochemical Assay
Reactivity
Human
708
494
192
78
31
19
17
15
13
11
9
9
7
7
5
4
4
4
2
2
Host
Mouse
509
390
166
16
6
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD11b antibody is conjugated to PE
152
123
92
52
20
19
15
15
14
14
14
13
11
10
9
9
9
8
7
7
7
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6
6
6
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
879
236
227
178
170
142
98
97
95
67
25
21
21
13
8
6
5
5
4
3
3
1
1
1
1
1
1
1
1
1
1
Options
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Clone ICRF44
Isotype IgG1
Characteristics The antibody detects: Human CD11b/Mac-1
Alternative Name CD11b/Mac-1a (ITGAM Antibody Abstract)
Background CD11b is the 165 kDa M chain which combines with the CD18 2 integrin subunit to form the Mac-1 heterodimer. It is expressed on activated lymphocytes, monocytes, granulocytes and a subset of NK cells. CD11b/Mac-1 functions in cell-cell and cell-substrate interactions and is a ligand for C3bi, CD50 (ICAM-3), CD54 (ICAM-1) and CD102 (ICAM-2).
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Application Notes Working Dilution:
FACS: 2 µL/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Blocking Studies

Restrictions For Research Use only
Buffer Product is supplied as 100 tests in 1.0 mL of PBS / NaN3 and a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Lee, Bankston, Arnaout, Liddington: "Two conformations of the integrin A-domain (I-domain): a pathway for activation?" in: Structure (London, England : 1993), Vol. 3, Issue 12, pp. 1333-40, 1996 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).