Lysosomal-Associated Membrane Protein 2 (LAMP2) antibody

Antigen: Lysosomal-Associated Membrane Protein 2 (LAMP2)

Synonyms: LAMPB, CD107b, LGP110, Mac3, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, MGC82091, LAMP2, MGC127297, lamp2, DKFZp459J126

Clonality: Monoclonal (H4B4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN135832

Additional Information

Alternative name: CD107b/LAMP-2

Isotype: IgG1

Clone: H4B4

Description: Human CD107b/LAMP-2 CD107b, also known as lysosomal-associated membrane protein 2 (LAMP-2), is a heavily glycosylated, type I transmembrane protein that constitutes the major sialoglycoproteins on lysosomal membranes. It is a ligand for galaptin, an S-type lectin present in extracellular matrix, through its recognition of acetyllactosamine oligosaccharide chains, and is a ligand for E-selectin-mediated cell adhesion. CD107b is expressed by granulocytes, T cells, macrophages, dendritic cells, activated platelets tonsillar epithelium and some tumor cell lines, including U937 and KG1a. It is also a widely expressed intracellular antigen. LAMP-2 may function in protecting the inner surface of the lysosomal membrane by forming a barrier to lysosomal hydrolases. The upregulation of both CD107a and CD107b on the surface of tumor cell lines has been associated with their enhanced metastatic potential, where they may increase adhesion to extracellular matrix and endothelium.

Application Details

Application Notes: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (10 L/10^6 cells), Biotin (10 L/10^6 cells), R-phycoerythrin (10 L/10^6 cells), Allophycocyanin (10 L/10^6 cells), Spectral Red (10 L/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Purification: Purified

Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Restrictions: For Research Use only

Publications

Febbraio, Silverstein: "Identification and characterization of LAMP-1 as an activation-dependent platelet surface glycoprotein." in: The Journal of biological chemistry, Vol. 265, Issue 30, pp. 18531-7, 1990 (PubMed).

Chen, Cha, Yuksel et al.: "Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. Sequence similarity to proteins bearing onco-differentiation antigens." in: The Journal of biological chemistry, Vol. 263, Issue 18, pp. 8754-8, 1988 (PubMed).

Sawada, Lowe, Fukuda: "E-selectin-dependent adhesion efficiency of colonic carcinoma cells is increased by genetic manipulation of their cell surface lysosomal membrane glycoprotein-1 expression levels." in: The Journal of biological chemistry, Vol. 268, Issue 17, pp. 12675-81, 1993 (PubMed).

Sawada, Jardine, Fukuda: "The genes of major lysosomal membrane glycoproteins, lamp-1 and lamp-2. 5'-flanking sequence of lamp-2 gene and comparison of exon organization in two genes." in: The Journal of biological chemistry, Vol. 268, Issue 12, pp. 9014-22, 1993 (PubMed).