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Progesterone Receptor antibody (PGR) (AA 1-101)

Details for Product anti-PGR Antibody No. ABIN136265, Supplier: Login to see
Antigen
Alternatives
anti-Human Progesterone Receptor antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
AA 1-101
76
53
45
35
32
27
18
18
17
16
16
15
13
9
8
8
8
7
7
6
6
6
5
5
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
578
66
64
13
6
5
4
3
2
2
1
Host
Mouse
333
256
4
Clonality (Clone)
Monoclonal ()
Conjugate
This Progesterone Receptor antibody is un-conjugated
16
15
13
8
8
8
3
2
2
2
2
2
2
2
2
1
Application
Western Blotting (WB)
412
163
141
137
105
56
46
33
24
9
7
3
3
3
3
2
2
1
1
1
1
1
Supplier
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Immunogen 6-His fusion protein containing the region encoding amino acids 1-101 of the human progesterone receptor expressed in E. coli.
Clone 4D8
Isotype IgG2a
Fragment Light chain,Chain kappa
Specificity This antibody recognizes both Form A (94 kDa) and Form B (120kDa) of the human progesterone receptor (PgR)
Characteristics Cellular Localization: Nuclear
Purification Protein G affinity purified. From hybridoma tissue culture supernatant.
Alternative Name Progesterone receptor (PGR Antibody Abstract)
Background The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins.
Synonyms: progesterone receptor PgR Anti-NR3C3 Anti-PGR Anti-PR Anti-Progesterone Receptor NR3C3 antibody PGR antibody PR antibody
Research Area Hormones, Receptors
Pathways Nuclear Receptor Transcription Pathway
Application Notes WB: Use at a concentration of 1-5 ug/ml. Predicted molecular weight: 99 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Comment

Positive Controls: MCF7 lysate (GTX23871) & recombinant protein

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Background publications Edwards: "Coregulatory proteins in nuclear hormone receptor action." in: Vitamins and hormones, Vol. 55, pp. 165-218, 1999 (PubMed).

Beato, Herrlich, Schütz: "Steroid hormone receptors: many actors in search of a plot." in: Cell, Vol. 83, Issue 6, pp. 851-7, 1996 (PubMed).

Evans: "The steroid and thyroid hormone receptor superfamily." in: Science (New York, N.Y.), Vol. 240, Issue 4854, pp. 889-95, 1988 (PubMed).

Bagchi, Elliston, Tsai et al.: "Steroid hormone-dependent interaction of human progesterone receptor with its target enhancer element." in: Molecular endocrinology (Baltimore, Md.), Vol. 2, Issue 12, pp. 1221-9, 1989 (PubMed).