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BRCA1 antibody (Breast Cancer 1) (AA 341-748)

Details for Product anti-BRCA1 Antibody No. ABIN136313, Supplier: Login to see
Antigen
Alternatives
anti-Human BRCA1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 341-748
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33
27
24
22
22
15
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11
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6
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Reactivity
Human
436
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33
4
1
Host
Mouse
381
59
5
3
Clonality (Clone)
Monoclonal ()
Conjugate
This BRCA1 antibody is un-conjugated
15
14
12
9
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6
2
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Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
262
119
105
75
72
68
51
14
9
7
3
2
2
1
1
1
Supplier
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Immunogen BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Clone 6B4
Isotype IgG1
Fragment Light chain,Chain kappa
Specificity This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
Cross-Reactivity Mouse (Murine)
Characteristics Cellular Localization: Nuclear
Purification Protein G purified. From mouse ascitic fluid.
Alternative Name BRCA1 / RNF53 (BRCA1 Antibody Abstract)
Background BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
Synonyms: 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4
Molecular Weight 220 kDa
Pathways Cell Division Cycle, DNA Damage Repair
Application Notes For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Comment

Positive Controls: HBL100

Restrictions For Research Use only
Format Liquid
Concentration 1.36 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, no preservative added
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Flow Cytometry (FACS) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody
Immunofluorescence (IF) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 2)
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 3)
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 4)
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 5)
Background publications Zheng, Annab, Afshari et al.: "BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 17, pp. 9587-92, 2001 (PubMed).

Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).