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BRCA1 antibody (Breast Cancer 1) (AA 341-748)

Details for Product anti-BRCA1 Antibody No. ABIN136313, Supplier: Login to see New
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Antigen
Alternatives
Epitope
AA 341-748
(36), (33), (26), (25), (22), (22), (15), (15), (15), (15), (14), (13), (12), (10), (10), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(443), (45), (34), (4), (1), (1), (1)
Host
Mouse
(390), (59), (5), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
This BRCA1 antibody is un-conjugated
(16), (14), (12), (9), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
(269), (121), (109), (81), (72), (68), (52), (17), (12), (7), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mg
Shipping to United States ( )
Immunogen BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Clone 6B4
Isotype IgG1
Fragment Light chain,Chain kappa
Specificity This antibody recognizes BRCA1, a 220-kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
Cross-Reactivity Mouse (Murine)
Characteristics Cellular Localization: Nuclear
Purification Protein G purified. From mouse ascitic fluid.
Alternative Name BRCA1 / RNF53 (BRCA1 Antibody Abstract)
Background BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
Synonyms: 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4
Molecular Weight 220 kDa
Pathways Cell Division Cycle, DNA Damage Repair
Application Notes For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Comment

Positive Controls: HBL100

Restrictions For Research Use only
Format Liquid
Concentration 1.36 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, no preservative added
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Flow Cytometry (FACS) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody
Immunofluorescence (IF) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 2)
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 3)
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 4)
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136313) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 5)
Background publications Zheng, Annab, Afshari et al.: "BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 17, pp. 9587-92, 2001 (PubMed).

Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).

Catalog No. ABIN136313
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