Breast Cancer 1 (BRCA1) antibody

Details for Product No. ABIN136313
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Antigen
Synonyms BRCA1
Reactivity
Human
(336), (45), (24), (13), (2), (1), (1), (1)
Host
Mouse
(296), (61), (3), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(14), (13), (11), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3)
Application
Immunoprecipitation (IP), Western Blotting (WB)
(194), (92), (90), (86), (69), (65), (50), (5), (5), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN136313
Quantity 0.1 mg
Price
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Immunogen BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Purification Protein G affinity purified
Alternative Name BRCA1 / RNF53
Background BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the ?exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.
Application Notes For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate-buffered saline, pH 7.2, no preservative added
General Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).

Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

Zheng, Annab, Afshari et al.: "BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 17, pp. 9587-92, 2001 (PubMed).

Hosts (296), (61), (3), (1)
Reactivities (336), (45), (24), (13), (2), (1), (1), (1)
Applications (194), (92), (90), (86), (69), (65), (50), (5), (5), (1), (1), (1)
Conjugates (14), (13), (11), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3)
Epitopes (41), (35), (24), (18), (16), (15), (14), (14), (13), (12), (10), (8), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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