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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Breast Cancer 1, Early Onset (BRCA1) antibody
|Synonyms||BRCA1, iris, pscp, brcai, brcc1, rnf53, brca1, LOC100224649, IRIS, PSCP, BRCAI, BRCC1, RNF53, BROVCA1, ARABIDOPSIS THALIANA BREAST CANCER SUSCEPTIBILITY1, ATBRCA1, breast cancer susceptibility1|
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Quantity||0.1 mg (1 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||BRCA1 / RNF53|
|Immunogen||BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||BRCA1-6B4 recognizes full length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the ?exon 11 splice variant. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Recent studies suggest a role for BRCA1 in DNA double strand break repair because of its association with Rad51.|
|Application Notes||For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.|
|Purification||Protein G affinity purified|
|Buffer||Phosphate-buffered saline, pH 7.2, no preservative added|
|Restrictions||For Research Use only|
Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).
Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).
Zheng, Annab, Afshari et al.: "BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 17, pp. 9587-92, 2001 (PubMed).