anti-CENPF antibody (Centromere Protein F) (AA 1759-2093)

Details for Product anti-CENPF Antibody No. ABIN136318
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Synonyms LOC100222985, 6530404A22Rik, AI325968, CENF, Lek1, PRO1779, mitosin, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, hcp-1, CMF1
AA 1759-2093
(21), (7), (2), (2), (2), (1), (1), (1)
(65), (3), (3), (3)
(32), (18), (16)
Clonality (Clone)
Monoclonal ()
This CENPF antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(25), (22), (17), (17), (14), (12), (10), (6), (4), (3), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States ( )
Immunogen Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
Clone 14C10 1D8
Isotype IgG2a
Fragment Light chain,Chain kappa
Characteristics Cellular Localization: Nuclear
Purification Protein G affinity purified.. From mouse ascitic fluid..
Alternative Name CENP-F (CENPF Antibody Abstract)
Background Mitosin-14C10 recognizes mitosin, a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Synonyms: CENF, PRO1779, hcp-1, 1063 CENPF CENP F AH Antigen, 1063 CENPF CENPF AH Antigen
Molecular Weight 350 kDa
Application Notes Western (1:500)

Positive Controls: HeLa (G2 phase)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Western Blotting (WB) image for anti-CENPF antibody (Centromere Protein F) (AA 1759-2093) (ABIN136318) anti-Centromere Protein F (CENPF) (AA 1759-2093) antibody
Background publications Clark, Allred, Hilsenbeck et al.: "Mitosin (a new proliferation marker) correlates with clinical outcome in node-negative breast cancer." in: Cancer research, Vol. 57, Issue 24, pp. 5505-8, 1998 (PubMed).

Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).

Catalog No. ABIN136318
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