Centromere Protein F (CENPF) (AA 1759-2093) antibody

Details for Product No. ABIN136318
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Antigen
Synonyms LOC100222985, 6530404A22Rik, AI325968, CENF, Lek1, PRO1779, mitosin, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, hcp-1, CMF1
Epitope
AA 1759-2093
(10), (4), (2), (2), (1), (1)
Reactivity
Human
(42), (2), (1), (1)
Host
Mouse
(27), (9), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(19), (16), (15), (10), (9), (6), (6), (4), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN136318
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
Clone 14C10 1D8
Isotype IgG2a
Fragment Light chain,Chain kappa
Characteristics Cellular Localization: Nuclear
Purification Protein G affinity purified.. From mouse ascitic fluid..
Alternative Name CENP-F
Background Mitosin-14C10 recognizes mitosin, a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Synonyms: CENF, PRO1779, hcp-1, 1063 CENPF CENP F AH Antigen, 1063 CENPF CENPF AH Antigen
Molecular Weight 350 kDa
Application Notes Western (1:500)
Comment

Positive Controls: HeLa (G2 phase)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).

Clark, Allred, Hilsenbeck et al.: "Mitosin (a new proliferation marker) correlates with clinical outcome in node-negative breast cancer." in: Cancer research, Vol. 57, Issue 24, pp. 5505-8, 1998 (PubMed).

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