Breast Cancer 1 (BRCA1) (AA 762-1315) antibody

Details for Product No. ABIN136329
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Antigen
Synonyms BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53
Epitope
AA 762-1315
(41), (36), (24), (22), (17), (16), (15), (14), (14), (14), (12), (8), (7), (7), (7), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(366), (45), (25), (13), (1), (1), (1), (1)
Host
Mouse
(325), (59), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (15), (12), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
(221), (95), (90), (88), (73), (68), (55), (12), (9), (3), (1), (1)
Pubmed 10 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN136329
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 762-1315.
Clone 17F8
Isotype IgG1
Fragment Light chain,Chain kappa
Specificity This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.
Cross-Reactivity Mouse (Murine)
Characteristics Cellular Localization: Nuclear/Cytoplasmic
Purification Protein G purified. From mouse ascitic fluid.
Alternative Name BRCA1 / RNF53
Background BRCA1-17F8 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein, and does not recognize the Aexon 11 splice variant. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Synonyms: 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4
Molecular Weight 220 kDa
Application Notes For IF: Use at a concentration of 1 ug/ml. For WB: Use at 1-10 ug/ml. Predicted molecular weight: 208 kDa. For IHC-P: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher
Comment

Positive Controls: 293T , A431 , HeLa , MCF7 , MEF

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH7.2 containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).

Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

Yoshikawa, Honda, Inamoto et al.: "Reduction of BRCA1 protein expression in Japanese sporadic breast carcinomas and its frequent loss in BRCA1-associated cases." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 5, Issue 6, pp. 1249-61, 1999 (PubMed).

Altiok, Batt, Altiok et al.: "Heregulin induces phosphorylation of BRCA1 through phosphatidylinositol 3-Kinase/AKT in breast cancer cells." in: The Journal of biological chemistry, Vol. 274, Issue 45, pp. 32274-8, 1999 (PubMed).

Arizti, Fang, Park et al.: "Tumor suppressor p53 is required to modulate BRCA1 expression." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7450-9, 2000 (PubMed).

Lu, Arrick: "Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms." in: Oncogene, Vol. 19, Issue 54, pp. 6351-60, 2001 (PubMed).

Bernard-Gallon, Dechelotte, Vissac et al.: "BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite." in: Breast cancer research : BCR, Vol. 3, Issue 1, pp. 61-5, 2001 (PubMed).

Zhong, Boyer, Chen et al.: "Deficient nonhomologous end-joining activity in cell-free extracts from Brca1-null fibroblasts." in: Cancer research, Vol. 62, Issue 14, pp. 3966-70, 2002 (PubMed).

Ongusaha, Ouchi, Kim et al.: "BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest." in: Oncogene, Vol. 22, Issue 24, pp. 3749-58, 2003 (PubMed).

Lee, Chew, Farmer et al.: "Biological functions of the BRCA1 protein." in: Breast disease, Vol. 10, Issue 1-2, pp. 11-22, 2005 (PubMed).

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