Breast Cancer 1 (BRCA1) (AA 341-748) antibody

Details for Product No. ABIN136331
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Antigen
Synonyms BRCAI, BRCC1, BROVCA1, IRIS, PNCA4, PPP1R53, PSCP, RNF53
Epitope
AA 341-748
(41), (36), (24), (22), (17), (16), (15), (14), (14), (14), (12), (8), (7), (7), (6), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(366), (45), (25), (13), (1), (1), (1), (1)
Host
Mouse
(325), (59), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (15), (12), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3), (1), (1), (1)
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(221), (95), (90), (89), (73), (68), (56), (13), (9), (3), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN136331
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 341-748.
Clone 8F7
Isotype IgG1
Fragment Light chain,Chain kappa
Specificity This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
Cross-Reactivity Mouse (Murine)
Characteristics Cellular Localization: Nuclear/Cytoplasmic
Purification Protein G purified. From Ascites.
Alternative Name BRCA1 / RNF53
Background BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Synonyms: 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4
Molecular Weight 220 kDa
Application Notes For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).

Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

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