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BRCA1 antibody (Breast Cancer 1) (AA 341-748)

Details for Product anti-BRCA1 Antibody No. ABIN136331, Supplier: Login to see
Antigen
Alternatives
anti-Human BRCA1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 341-748
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Reactivity
Human
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Host
Mouse
381
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3
Clonality (Clone)
Monoclonal ()
Conjugate
This BRCA1 antibody is un-conjugated
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
262
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68
51
14
9
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3
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2
1
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1
Supplier
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 341-748.
Clone 8F7
Isotype IgG1
Fragment Light chain,Chain kappa
Specificity This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. Mutations in this tumor suppressor gene greatly increase the risk of breast cancer.
Cross-Reactivity Mouse (Murine)
Characteristics Cellular Localization: Nuclear/Cytoplasmic
Purification Protein G purified. From Ascites.
Alternative Name BRCA1 / RNF53 (BRCA1 Antibody Abstract)
Background BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Synonyms: 113705, RNF53, BRCA1, BRCAI, 672, PSCP, BRCC1, P38398, IRIS, BROVCA1, PNCA4
Molecular Weight 220 kDa
Pathways Cell Division Cycle, DNA Damage Repair
Application Notes For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Immunohistochemistry (IHC) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136331) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody
Western Blotting (WB) image for anti-BRCA1 antibody (Breast Cancer 1) (AA 341-748) (ABIN136331) anti-Breast Cancer 1 (BRCA1) (AA 341-748) antibody (Image 2)
Background publications Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).