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Details for Product No. ABIN136331

Breast Cancer 1 (BRCA1) antibody

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Antigen
Synonyms BRCA1
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN136331
Quantity 0.1 mg
Price
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 341-748.
Isotype IgG1
Purification Protein G affinity purified
Alternative Name BRCA1 / RNF53
Background BRCA1-8F7 recognizes full-length BRCA1, a 220kDa nuclear phosphoprotein. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm. Its usefulness to monitor functional inactivation of BRCA1 in sporadic breast cancers is under active investigation.
Application Notes For WB: Use at a concentration of 1-10ug/ml. This antibody recognizes full-length BRCA1, a 220-kDa nuclear phosphoprotein. For IP: Use at an assay dependent dilution. For IHC: Use at an assay dependent dilution. Block endogenous peroxidase with 0.3% H2O2 for 30 minutes. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (0.1 M citrate buffer at 95C in water bath for 20 min). Avoid nonspecific binding by incubating in normal rabbit anti-mouse serum. Incubate primary antibody overnight and use a biotin-streptavidin amplification kit. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Chen, Chen, Riley et al.: "Aberrant subcellular localization of BRCA1 in breast cancer." in: Science (New York, N.Y.), Vol. 270, Issue 5237, pp. 789-91, 1995 (PubMed).

Chen, Farmer, Chen et al.: "BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner." in: Cancer research, Vol. 56, Issue 14, pp. 3168-72, 1996 (PubMed).

Alternatives for antigen "Breast Cancer 1 (BRCA1)", type "Antibodies"
Hosts (295), (80), (4), (1)
Reactivities (357), (35), (24), (13), (2), (2), (1), (1), (1), (1)
Applications (217), (95), (90), (84), (72), (70), (63), (4), (2), (2), (1), (1), (1)
Conjugates (16), (16), (10), (9), (9), (9), (9), (9), (9), (9), (9), (6), (3), (3), (1)
Epitopes (37), (33), (24), (17), (16), (15), (15), (14), (13), (12), (12), (7), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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