Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) antibody

Antigen: Damage-Specific DNA Binding Protein 1, 127kDa (DDB1)

Synonyms: XPE, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB1, MGC143388, xpe, ddba, xap1, xpce, xpe-bf, uv-ddb1, DKFZp459H0524

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN136432

Additional Information

Alternative name: DDB1 / XAP1

Immunogen: Synthetic peptide conugated to protein carrier. The mice were immunized with a combination of N & C peptide. The Swiss Prot ID is Q16531. The exact region recognized by this product has not yet been determined.

Cross-Reactivity: Human

Isotype: IgG

Description: The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

Specificity: Human DDB1. Other species have not been tested.

Application Details

Application Notes: 1/1000 in PBS/Milk 5%/Tween 0,05%. Incubation OVN at 4 C. Secondary antibody 2 hours at room temperature with dilution 1/5000 in PBS/Milk 5%/Tween 0,05%. ECL.

Concentration: batch dependent

Purification: Protein G affinity purified

Buffer: Phosphate-buffered saline, pH 7.2, no preservative added.

Restrictions: For Research Use only

Images

anti-Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) antibody

Publications

Holschneider: "[Current interpretation of the testicular maldescention problem]" in: Tierärztliche Praxis, Vol. 3, Issue 1, pp. 123-7, 1976 (PubMed).

Chu, Chang: "Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA." in: Science (New York, N.Y.), Vol. 242, Issue 4878, pp. 564-7, 1988 (PubMed).