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Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) antibody

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Antigen
Alternatives
Reactivity
Human
(89), (31), (26), (6), (4), (3), (2), (1), (1), (1), (1), (1)
Host
Mouse
(48), (24), (18)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(83), (39), (26), (23), (15), (12), (5), (4), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Quantity 0.1 mg
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Immunogen Synthetic peptide congugated to protein carrier. The mice were immunized with a combination of N & C peptide. The Swiss Prot ID is Q16531.
Clone 1322
Isotype IgG
Specificity Human DDB1. Other species have not been tested.
Characteristics Cellular Localization: Nuclear
Purification Protein G affinity purified. From mouse ascitic fluid.
Alternative Name DDB1 / XAP1 (DDB1 Antibody Abstract)
Background The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.
Synonyms: DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF, XPE BF, UV DDB1, XPEBF, UVDDB1
Molecular Weight 130 kDa
Pathways DNA Damage Repair
Application Notes 1/1000 in PBS/Milk 5%/Tween 0,05%. Incubation OVN at 4 C. Secondary antibody 2 hours at room temperature with dilution 1/5000 in PBS/Milk 5%/Tween 0,05%. ECL.
Comment

Positive Controls: T47D and MCF-7 whole cell lysate

Restrictions For Research Use only
Format Liquid
Concentration 3 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment For long term storage, please keep at -70 °C or below.
Supplier Images
Image no. 1 for anti-Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) antibody (ABIN136432) anti-Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) antibody
Background publications Chu, Chang: "Xeroderma pigmentosum group E cells lack a nuclear factor that binds to damaged DNA." in: Science (New York, N.Y.), Vol. 242, Issue 4878, pp. 564-7, 1988 (PubMed).

Holschneider: "[Current interpretation of the testicular maldescention problem]" in: Tierärztliche Praxis, Vol. 3, Issue 1, pp. 123-7, 1976 (PubMed).

Catalog No. ABIN136432
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