Checkpoint Kinase 2 (CHEK2) antibody

Antigen: Checkpoint Kinase 2 (CHEK2)

Synonyms: CDS1, CHK2, LFS2, RAD53, HuCds1, PP1425

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN137205

Additional Information

Alternative name: CHK2

Immunogen: Recombinant CHK2 protein

Cross-Reactivity: Human

Isotype: IgG

Description: CHK2 controls cell cycle checkpoint and may participate in transduction of the DNA damage and replicational stress signals. CHK2 belongs to the Ser/Thr protein kinase family and its kinase actibity is up-regulated by autophosphorylation, It is phosphorylated rapidly in response to DNA damage and to replication block.

Specificity: ABIN137205 is specific for human CHK2 protein.

Application Details

Application Notes: For western blot, use 1:1000 dilution to detect CHK2 protein in ~20-40 ug total cell extract.

Concentration: 1 mg/ml

Purity: >95% by SDS-PAGE

Purification: Protein G affinity purified

Buffer: Phosphate-buffered saline, pH 7.2, no preservative added.

Preservative: containing no preservatives

Storage: For long term storage, please keep at -70 C or below. Avoid repeared freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-Checkpoint Kinase 2 (CHEK2) antibody

Publications

Brody: "CHEKs and balances: accounting for breast cancer." in: Nature genetics, Vol. 31, Issue 1, pp. 3-4, 2002 (PubMed).

Ahn, Urist, Prives: "Questioning the role of checkpoint kinase 2 in the p53 DNA damage response." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20480-9, 2003 (PubMed).

Jallepalli, Lengauer, Vogelstein et al.: "The Chk2 tumor suppressor is not required for p53 responses in human cancer cells." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20475-9, 2003 (PubMed).

Stevens, Smith, La Thangue: "Chk2 activates E2F-1 in response to DNA damage." in: Nature cell biology, Vol. 5, Issue 5, pp. 401-9, 2003 (PubMed).

Wu, Chen: "Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36163-8, 2003 (PubMed).

Tan, Guschin, Davalos et al.: "Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 21, pp. 11997-2002, 2003 (PubMed).

Lipton, Fleischmann, Sieber et al.: "Contribution of the CHEK2 1100delC variant to risk of multiple colorectal adenoma and carcinoma." in: Cancer letters, Vol. 200, Issue 2, pp. 149-52, 2003 (PubMed).