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Checkpoint Kinase 2 (CHEK2) antibody

Details for Product No. ABIN137205, Supplier: Login to see
Antigen
Alternatives
anti-Human Checkpoint Kinase 2 antibody for Enzyme Immunoassay
Reactivity
Human
419
160
160
8
8
6
5
4
1
1
1
Host
Mouse
335
89
8
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
6
6
5
5
5
5
5
5
5
5
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
341
115
107
70
67
57
37
29
24
6
5
2
1
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Immunogen Recombinant CHK2 protein
Clone 8F12
Isotype IgG
Specificity Product is specific for human CHK2 protein.
Characteristics Cellular Localization: Nuclear
Purification Protein G affinity purified
Purity > 95 % by SDS-PAGE
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background CHK2 controls cell cycle checkpoint and may participate in transduction of the DNA damage and replicational stress signals. CHK2 belongs to the Ser/Thr protein kinase family and its kinase actibity is up-regulated by autophosphorylation, It is phosphorylated rapidly in response to DNA damage and to replication block.
Synonyms: HuCds1, 114480, CHK2, CDS1 CHK2 HuCds1 PP1425 RAD53 bA444G7 checkpoint kinase 2 checkpoint-like protein CHK2 11200, PP1425, CDS1 CHK2 HuCds1 PP1425 RAD53 bA444G7 checkpoint kinase2 checkpointlike protein CHK211200, CDS1 CHK2 HuCds1 PP1425 RAD53 bA444G7 checkpoint kinase 2 checkpointlike protein CHK2 11200, LFS2, RAD53, O96017, CHEK2, CDS1
Molecular Weight ~61 kD
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes For western blot, use 1:1000 dilution to detect CHK2 protein in ~20-40 ug total cell extract.
Comment

Positive Controls: HepG2, MCF-7, or LN-Cap total cell extract

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
 image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN137205) anti-Checkpoint Kinase 2 (CHEK2) antibody
Background publications Lipton, Fleischmann, Sieber et al.: "Contribution of the CHEK2 1100delC variant to risk of multiple colorectal adenoma and carcinoma." in: Cancer letters, Vol. 200, Issue 2, pp. 149-52, 2003 (PubMed).

Tan, Guschin, Davalos et al.: "Zinc-finger protein-targeted gene regulation: genomewide single-gene specificity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 21, pp. 11997-2002, 2003 (PubMed).

Wu, Chen: "Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36163-8, 2003 (PubMed).

Stevens, Smith, La Thangue: "Chk2 activates E2F-1 in response to DNA damage." in: Nature cell biology, Vol. 5, Issue 5, pp. 401-9, 2003 (PubMed).

Jallepalli, Lengauer, Vogelstein et al.: "The Chk2 tumor suppressor is not required for p53 responses in human cancer cells." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20475-9, 2003 (PubMed).

Ahn, Urist, Prives: "Questioning the role of checkpoint kinase 2 in the p53 DNA damage response." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20480-9, 2003 (PubMed).

Brody: "CHEKs and balances: accounting for breast cancer." in: Nature genetics, Vol. 31, Issue 1, pp. 3-4, 2002 (PubMed).