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CENPF antibody (Centromere Protein F) (AA 1759-2093)

Details for Product anti-CENPF Antibody No. ABIN138239, Supplier: Login to see
Antigen
Epitope
AA 1759-2093
23
9
2
2
1
1
1
1
Reactivity
Human
57
3
3
3
1
Host
Mouse
21
19
18
Clonality (Clone)
Monoclonal ()
Conjugate
This CENPF antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
24
21
19
18
14
14
6
5
3
2
1
1
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
Clone 7F11-2
Isotype IgG2a
Fragment Light chain,Chain kappa
Purification Protein G affinity purified. From mouse ascitic fluid.
Alternative Name CENP-F (CENPF Antibody Abstract)
Background Mitosin is a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Synonyms: CENF, PRO1779, hcp-1
Molecular Weight 350 kDa
Pathways
Application Notes Immunohistochemical studies were performed at dilutions of 10 g/mL. Briefly, 4-m tissue sections were cut from the same blocks of hematoxylin and eosin-stained slides, mounted on charged slides, deparaffinized in xylene, and rehydrated in descending grades of ethyl alcohol (from 100% to 70%). The slides used for mitosin staining were then subjected to heat-induced antigen retrieval by immersion in a 0.01 mol/L citrate buffer, pH 6.0, preheated to > 90 C, and heated in an electric vegetable steamer (Black and Decker, Shelton, CT) for 54 minutes. The endogenous peroxidase activity was blocked by a 5-minute treatment with 3% hydrogen peroxide in absolute methanol. Immunohistochemical analysis was then performed using the avidin-biotin technique (LSAB2 peroxidase kit, DAKO, Carpinteria, CA). The antigen-antibody immunoreaction was visualized using 3-3-diaminobenzedine as the chromogen, and the slides were counterstained with Mayer hematoxylin.
Comment

Positive Controls: HeLa

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
 image for anti-CENPF antibody (Centromere Protein F) (AA 1759-2093) (ABIN138239) anti-Centromere Protein F (CENPF) (AA 1759-2093) antibody
Background publications Clark, Allred, Hilsenbeck et al.: "Mitosin (a new proliferation marker) correlates with clinical outcome in node-negative breast cancer." in: Cancer research, Vol. 57, Issue 24, pp. 5505-8, 1998 (PubMed).

Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).