anti-CENPF antibody (Centromere Protein F) (AA 1759-2093)

Details for Product anti-CENPF Antibody No. ABIN138239
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Synonyms LOC100222985, 6530404A22Rik, AI325968, CENF, Lek1, PRO1779, mitosin, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, hcp-1, CMF1
AA 1759-2093
(21), (7), (2), (2), (2), (1), (1), (1)
(65), (3), (3), (3)
(32), (18), (16)
Clonality (Clone)
Monoclonal ()
This CENPF antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
(26), (22), (18), (17), (15), (11), (10), (6), (5), (3), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
Clone 7F11-2
Isotype IgG2a
Fragment Light chain,Chain kappa
Purification Protein G affinity purified. From mouse ascitic fluid.
Alternative Name CENP-F (CENPF Antibody Abstract)
Background Mitosin is a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Synonyms: CENF, PRO1779, hcp-1
Molecular Weight 350 kDa
Application Notes Immunohistochemical studies were performed at dilutions of 10 g/mL. Briefly, 4-m tissue sections were cut from the same blocks of hematoxylin and eosin-stained slides, mounted on charged slides, deparaffinized in xylene, and rehydrated in descending grades of ethyl alcohol (from 100% to 70%). The slides used for mitosin staining were then subjected to heat-induced antigen retrieval by immersion in a 0.01 mol/L citrate buffer, pH 6.0, preheated to > 90 C, and heated in an electric vegetable steamer (Black and Decker, Shelton, CT) for 54 minutes. The endogenous peroxidase activity was blocked by a 5-minute treatment with 3% hydrogen peroxide in absolute methanol. Immunohistochemical analysis was then performed using the avidin-biotin technique (LSAB2 peroxidase kit, DAKO, Carpinteria, CA). The antigen-antibody immunoreaction was visualized using 3-3-diaminobenzedine as the chromogen, and the slides were counterstained with Mayer hematoxylin.

Positive Controls: HeLa

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate-buffered saline, pH 7.2, containing no preservatives
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Image no. 1 for anti-CENPF antibody (Centromere Protein F) (AA 1759-2093) (ABIN138239) anti-Centromere Protein F (CENPF) (AA 1759-2093) antibody
Background publications Clark, Allred, Hilsenbeck et al.: "Mitosin (a new proliferation marker) correlates with clinical outcome in node-negative breast cancer." in: Cancer research, Vol. 57, Issue 24, pp. 5505-8, 1998 (PubMed).

Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).

Validation Images
Catalog No. ABIN138239
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