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Details for Product No. ABIN138239

Centromere Protein F (CENPF) antibody

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Antigen
Synonyms CENF, hcp-1, PRO1779, CMF1, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, Lek1, mitosin, AI325968, 6530404A22Rik, LOC100222985
Reactivity
»Alternatives Human
Host
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (IHC)
Pubmed 2 references available
Catalog no. ABIN138239
Quantity 0.1 mg
Price
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Immunogen Protein fragment expressed in E. coli corresponding to amino acids 1759-2093.
Isotype IgG2a
Purification Protein G affinity purified
Alternative Name CENP-F
Background Mitosin is a 350kDa phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase, and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
Application Notes Immunohistochemical studies were performed at dilutions of 10 g/mL. Briefly, 4-m tissue sections were cut from the same blocks of hematoxylin and eosin-stained slides, mounted on charged slides, deparaffinized in xylene, and rehydrated in descending grades of ethyl alcohol (from 100% to 70%). The slides used for mitosin staining were then subjected to heat-induced antigen retrieval by immersion in a 0.01 mol/L citrate buffer, pH 6.0, preheated to > 90 C, and heated in an electric vegetable steamer (Black and Decker, Shelton, CT) for 54 minutes. The endogenous peroxidase activity was blocked by a 5-minute treatment with 3% hydrogen peroxide in absolute methanol. Immunohistochemical analysis was then performed using the avidin-biotin technique (LSAB2 peroxidase kit, DAKO, Carpinteria, CA). The antigen-antibody immunoreaction was visualized using 3-3-diaminobenzedine as the chromogen, and the slides were counterstained with Mayer hematoxylin.
Restrictions For Research Use only
Concentration 1
Buffer Phosphate-buffered saline, pH 7.2, no preservative added.
Storage 4 °C
Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).

Clark, Allred, Hilsenbeck et al.: "Mitosin (a new proliferation marker) correlates with clinical outcome in node-negative breast cancer." in: Cancer research, Vol. 57, Issue 24, pp. 5505-8, 1998 (PubMed).

Alternatives for antigen "Centromere Protein F (CENPF)", type "Antibodies"
Hosts (29), (12), (7)
Reactivities (44), (1), (1), (1), (1), (1)
Applications (27), (19), (16), (11), (9), (8), (6), (2), (1), (1)
Conjugates (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (9), (4)
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