CD130/gp130 antibody

Catalog No. ABIN1383683

Product Details anti-CD130/gp130 Antibody

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD130/gp130 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: Recognises the Gp130, common subunit for IL-6, IL-11, OSM, LIF, CNTF, CT-1 receptors, a 130-140 kDa protein

Purification: Ion exchange chromatography

Sterility: 0.22 μm filtered

Immunogen: Natural soluble gp130,Myeloma X63/AG.8653 x Balb/c spleen cells

Clone: B-P4

Isotype: IgG1 kappa

Biological Activity Comment: Inhibits IL-11 activity and binding to gp130, but not CNTF, LIF and OSM activities

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered saline. Sterile-filtered through 0.22 μm. Carrier and preservative free

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Stable at +2-8°C for 12 months. For longer storage freeze aliquots.

References for anti-CD130/gp130 antibody (ABIN1383683)

Waetzig, Chalaris, Rosenstiel, Suthaus, Holland, Karl, Vallés Uriarte, Till, Scheller, Grötzinger, Schreiber, Rose-John, Seegert: "N-linked glycosylation is essential for the stability but not the signaling function of the interleukin-6 signal transducer glycoprotein 130." in: The Journal of biological chemistry, Vol. 285, Issue 3, pp. 1781-9, (2010) (PubMed).

Adam, Rabe, Suthaus, Grötzinger, Rose-John, Scheller: "Unraveling viral interleukin-6 binding to gp130 and activation of STAT-signaling pathways independently of the interleukin-6 receptor." in: Journal of virology, Vol. 83, Issue 10, pp. 5117-26, (2009) (PubMed).

Tenhumberg, Waetzig, Chalaris, Rabe, Seegert, Scheller, Rose-John, Grötzinger: "Structure-guided optimization of the interleukin-6 trans-signaling antagonist sgp130." in: The Journal of biological chemistry, Vol. 283, Issue 40, pp. 27200-7, (2008) (PubMed).

Giese, Roderburg, Sommerauer, Wortmann, Metz, Heinrich, Müller-Newen: "Dimerization of the cytokine receptors gp130 and LIFR analysed in single cells." in: Journal of cell science, Vol. 118, Issue Pt 21, pp. 5129-40, (2005) (PubMed).

Buk, Waibel, Braig, Martens, Heinrich, Graeve: "Polarity and lipid raft association of the components of the ciliary neurotrophic factor receptor complex in Madin-Darby canine kidney cells." in: Journal of cell science, Vol. 117, Issue Pt 10, pp. 2063-75, (2004) (PubMed).

Classen-Linke, Müller-Newen, Heinrich, Beier, von Rango: "The cytokine receptor gp130 and its soluble form are under hormonal control in human endometrium and decidua." in: Molecular human reproduction, Vol. 10, Issue 7, pp. 495-504, (2004) (PubMed).

Selander, Li, Watson, Merrell, Dahmen, Heinrich, Müller-Newen, Harris: "Inhibition of gp130 signaling in breast cancer blocks constitutive activation of Stat3 and inhibits in vivo malignancy." in: Cancer research, Vol. 64, Issue 19, pp. 6924-33, (2004) (PubMed).

Giese, Au-Yeung, Herrmann, Diefenbach, Haan, Küster, Wortmann, Roderburg, Heinrich, Behrmann, Müller-Newen: "Long term association of the cytokine receptor gp130 and the Janus kinase Jak1 revealed by FRAP analysis." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 39205-13, (2003) (PubMed).

Timmermann, Küster, Kurth, Heinrich, Müller-Newen: "A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor." in: European journal of biochemistry / FEBS, Vol. 269, Issue 11, pp. 2716-26, (2002) (PubMed).

Friederichs, Schmitz, Weissenbach, Heinrich, Schaper: "Interleukin-6-induced proliferation of pre-B cells mediated by receptor complexes lacking the SHP2/SOCS3 recruitment sites revisited." in: European journal of biochemistry / FEBS, Vol. 268, Issue 24, pp. 6401-7, (2001) (PubMed).

Aoki, Narazaki, Kishimoto, Tosato: "Receptor engagement by viral interleukin-6 encoded by Kaposi sarcoma-associated herpesvirus." in: Blood, Vol. 98, Issue 10, pp. 3042-9, (2001) (PubMed).

Kurth, Horsten, Pflanz, Timmermann, Küster, Dahmen, Tacken, Heinrich, Müller-Newen: "Importance of the membrane-proximal extracellular domains for activation of the signal transducer glycoprotein 130." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 1, pp. 273-82, (2000) (PubMed).

Schmitz, Dahmen, Grimm, Gendo, Müller-Newen, Heinrich, Schaper: "The cytoplasmic tyrosine motifs in full-length glycoprotein 130 have different roles in IL-6 signal transduction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 2, pp. 848-54, (2000) (PubMed).

Terstegen, Gatsios, Bode, Schaper, Heinrich, Graeve: "The inhibition of interleukin-6-dependent STAT activation by mitogen-activated protein kinases depends on tyrosine 759 in the cytoplasmic tail of glycoprotein 130." in: The Journal of biological chemistry, Vol. 275, Issue 25, pp. 18810-7, (2000) (PubMed).

Schmitz, Weissenbach, Haan, Heinrich, Schaper: "SOCS3 exerts its inhibitory function on interleukin-6 signal transduction through the SHP2 recruitment site of gp130." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12848-56, (2000) (PubMed).

Anhuf, Weissenbach, Schmitz, Sobota, Hermanns, Radtke, Linnemann, Behrmann, Heinrich, Schaper: "Signal transduction of IL-6, leukemia-inhibitory factor, and oncostatin M: structural receptor requirements for signal attenuation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 5, pp. 2535-43, (2000) (PubMed).

Pflanz, Kurth, Grötzinger, Heinrich, Müller-Newen: "Two different epitopes of the signal transducer gp130 sequentially cooperate on IL-6-induced receptor activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 12, pp. 7042-9, (2000) (PubMed).

Müller-Newen, Küster, Wijdenes, Schaper, Heinrich: "Studies on the interleukin-6-type cytokine signal transducer gp130 reveal a novel mechanism of receptor activation by monoclonal antibodies." in: The Journal of biological chemistry, Vol. 275, Issue 7, pp. 4579-86, (2000) (PubMed).

Montero-Julian, Brailly, Sautès, Joyeux, Dorval, Mosseri, Yasukawa, Wijdenes, Adler, Gorin, Fridman, Tartour: "Characterization of soluble gp130 released by melanoma cell lines: A polyvalent antagonist of cytokines from the interleukin 6 family." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 3, Issue 8, pp. 1443-51, (1999) (PubMed).

Kurth, Horsten, Pflanz, Dahmen, Küster, Grötzinger, Heinrich, Müller-Newen: "Activation of the signal transducer glycoprotein 130 by both IL-6 and IL-11 requires two distinct binding epitopes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 3, pp. 1480-7, (1999) (PubMed).

Target Details for CD130/gp130

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Alternative Name: CD130 (IL6ST Products)

Synonyms: CD130 antibody, CDW130 antibody, GP130 antibody, IL-6RB antibody, 5133400A03Rik antibody, AA389424 antibody, BB405851 antibody, D13Ertd699e antibody, gp130 antibody, Ac1055 antibody, Gp130 antibody, Il-6rb antibody, IL-6 antibody, interleukin 6 signal transducer antibody, IL6ST antibody, Il6st antibody, il6st antibody

Background: Gp130

Molecular Weight: 130-140 kDa

Pathways: JAK-STAT Signaling, Cellular Glucan Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Cancer Immune Checkpoints