Osteocalcin antibody (AA 21-100)

Catalog No. ABIN1385851

Product Details anti-Osteocalcin Antibody

Target: Osteocalcin (BGLAP)

Binding Specificity: AA 21-100

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteocalcin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Osteocalcin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Osteocalcin antibody (ABIN1385851)

Jiang, Zhou, Huang, Wu, Huang, Liang, Liu, Zheng, Zhao: "Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 6, pp. 2327-39, (2016) (PubMed).

Li, Zhou, Wang, Yang, Ding, Zhou: "Controlled dual delivery of BMP-2 and dexamethasone by nanoparticle-embedded electrospun nanofibers for the efficient repair of critical-sized rat calvarial defect." in: Biomaterials, Vol. 37, pp. 218-29, (2015) (PubMed).

Ding, Li, Liu, Yang, Zhou, Zhou: "A nano-micro alternating multilayer scaffold loading with rBMSCs and BMP-2 for bone tissue engineering." in: Colloids and surfaces. B, Biointerfaces, Vol. 133, pp. 286-95, (2015) (PubMed).

Target Details for Osteocalcin

Target: Osteocalcin (BGLAP)

Alternative Name: Osteocalcin (BGLAP Products)

Synonyms: BGP antibody, OC antibody, OCN antibody, Bglap1 antibody, Bglap2 antibody, OG1 antibody, mOC-A antibody, Bgp antibody, Bgpr antibody, Bgpra antibody, bgp antibody, LOC792433 antibody, ocn antibody, GLA antibody, BGLAP antibody, PMF1 antibody, bone gamma-carboxyglutamate protein antibody, bone gamma carboxyglutamate protein antibody, bone gamma-carboxyglutamate protein S homeolog antibody, bone gamma-carboxyglutamate (gla) protein antibody, osteocalcin antibody, polyamine-modulated factor 1 antibody, BGLAP antibody, Bglap antibody, bglap.S antibody, bglap antibody, ocn antibody, LOC100529177 antibody, LOC100146589 antibody

Background:

Synonyms: Osteocalcin, Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein, BGLAP

Background: Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2 % of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Gene ID: 632

UniProt: P02818