CSTF2 antibody (pSer498)
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- Target See all CSTF2 Antibodies
- CSTF2 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2))
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Binding Specificity
- pSer498
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CSTF2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Cow,Sheep,Pig,Horse
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human CstF64 around the phosphorylation site of Ser498
- Isotype
- IgG
- Top Product
- Discover our top product CSTF2 Primary Antibody
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- CSTF2 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2))
- Alternative Name
- CstF64 (CSTF2 Products)
- Synonyms
- CSTF2 antibody, CstF-64 antibody, fb11e07 antibody, zgc:56346 antibody, zgc:77730 antibody, wu:fb11e07 antibody, 64kDa antibody, C630034J23Rik antibody, Cstf64 antibody, CSFT64 antibody, cstF-64 antibody, cstf-64 antibody, cleavage stimulation factor subunit 2 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 2 antibody, cleavage stimulation factor, 3' pre-RNA subunit 2 antibody, CSTF2 antibody, cstf2 antibody, Cstf2 antibody, cstf2.L antibody
- Background
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Synonyms: p-CstF-64 Ser498, p-CstF-64 S498, CstF64 Ser498, CstF64 S498, CSTF2T, CF1 64 kDa subunit, tau variant, Cleavage stimulation factor 64 kDa subunit, tau variant, CSTF 64 kDa subunit, tau variant, KIAA0689, TauCstF64, CSTF2_HUMAN.
Background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.
- Gene ID
- 1478
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