CSTF2 antibody (pSer498)

Catalog No. ABIN1386863

Product Details anti-CSTF2 Antibody

Target: CSTF2 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2))

Binding Specificity: pSer498

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Sheep,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CstF64 around the phosphorylation site of Ser498

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CSTF2

Target: CSTF2 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 2, 64kDa (CSTF2))

Alternative Name: CstF64 (CSTF2 Products)

Synonyms: CSTF2 antibody, CstF-64 antibody, fb11e07 antibody, zgc:56346 antibody, zgc:77730 antibody, wu:fb11e07 antibody, 64kDa antibody, C630034J23Rik antibody, Cstf64 antibody, CSFT64 antibody, cstF-64 antibody, cstf-64 antibody, cleavage stimulation factor subunit 2 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 2 antibody, cleavage stimulation factor, 3' pre-RNA subunit 2 antibody, CSTF2 antibody, cstf2 antibody, Cstf2 antibody, cstf2.L antibody

Background:

Synonyms: p-CstF-64 Ser498, p-CstF-64 S498, CstF64 Ser498, CstF64 S498, CSTF2T, CF1 64 kDa subunit, tau variant, Cleavage stimulation factor 64 kDa subunit, tau variant, CSTF 64 kDa subunit, tau variant, KIAA0689, TauCstF64, CSTF2_HUMAN.

Background: Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.

Gene ID: 1478